不良孕产史人群中血栓前状态易感基因分布调查  

Investigation on the distribution of prethrombotic state susceptibility genes in the population with poor pregnancy

作　　者：张秀卿[1] 邓洋[2] 鹿静[2] 杨爱玲[2] 赵卉[2] 王晓兰[2] ZHANG Xiuqing;DENG Yang;LU Jing;YANG Ailing;ZHAO Hui;WANG Xiaolan(Department of Family Planning and Surgery,Taiyuan Maternal and Child Health Hospital,Taiyuan Shanxi 030012,China)

机构地区：[1]山西省太原市妇幼保健院计划生育手术科,030012 [2]山西省太原市妇幼保健院妇科

出　　处：《中国妇产科临床杂志》2022年第3期242-244,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：吴阶平医学基金会临床科研专项资助(320.6750.18558)。

摘　　要：目的 探究血栓前状态易感基因多态性在不良孕产史人群中的分布。方法 收集2018年12月至2021年6月在山西省太原市妇幼就诊的410例有不良孕产史的女性(病例组)和111例无不良孕产史备孕女性(对照组)作为研究对象。采集两组人群EDTA抗凝全血,提取其基因组DNA,利用荧光定量PCR技术检测血栓前状态易感基因多态性在两组人群中的分布。结果 (1) MTHFR A1298 C、PAI-1 5G/4G、FⅤA1691G、FⅡG20210A基因多态性在对照组和病例组中分布差异无统计学意义(P> 0.05);(2) MTRR A66G位点AA、AG、GG基因型在对照组和病例组中分布差异有统计学意义(P <0.05);(3) MTHFR C677T位点AA、AG、GG基因型及G、A等位基因频率在对照组和病例组中的分布差异有统计学意义(P <0.05)。结论 血栓前状态易感基因MTRR A66G位点、MTHFRC677T位点多态性与不良孕产史密切相关。Objective To explore the distribution of prethrombotic state susceptibility gene polymorphisms in the population with a history of poor pregnancy. Methods From December 2018 to June 2021, 410 women with a history of poor pregnancy and childbirth(research group) and 111 normal women(control group) were collected as the research subjects. EDTA anticoagulated whole blood of the two groups were collected,its genomic DNA were extracted,fluorescence quantitative PCR technology was used to detect the distribution of prethrombotic state susceptibility gene polymorphisms. Results(1) There was no significantly difference in MTHFR A1298 C, PAI-1 5G/4G, F Ⅴ A1691G,F Ⅱ G20210A gene polymorphisms between the two group(P > 0.05);(2) There were significantly difference in the distribution of genotypes in MTRR A66G sites AA,AG,GG between the two group(P < 0.05).(3) There were significantly difference in the AA,AG,GG genotypes and the G, A allele frequencies at the C677T site of MTHFR between the two group(P < 0.05). Conclusion The prethrombotic state susceptibility gene MTRR A66G locus and MTHFR C677T locus polymorphisms are related to the history of adverse pregnancy and childbirth.

关 键 词：血栓前状态 基因多态性 不良孕产史 基因型 

分 类 号：R714[医药卫生—妇产科学]