3D基因组结构调控蛋白质的相分离特征分析  

Characterization of phase separation in 3D genome organization

作　　者：邢佳庆 李腾 李卫华 李亭亭 XING Jia-qing;LI Teng;LI Wei-hua;LI Ting-ting(National Center for Biomedical Analysis,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]军事科学院军事医学研究院生物医学分析中心,北京100850

出　　处：《军事医学》2022年第3期161-166,共6页Military Medical Sciences

基　　金：国家自然科学基金优秀青年科学基金项目(81922051)。

摘　　要：目的系统分析相分离和3D基因组结构调控蛋白质之间的关系。方法通过预测所有氨基酸长度>100的人类蛋白质的内在固有无序序列(IDR),进行基于IDR比例的基因集富集分析(GESA),并筛选受相分离调控的3D基因组相关生物过程。结果总共19843个人类蛋白质中有3885个蛋白质富含IDR,其中1427个参与调控3D基因组结构。这些富含IDR并参与3D基因组组织结构的蛋白质具有氨基酸种类和模块结构域偏好性。研究开发Phasepro Shiny应用程序,能够分析和可视化蛋白质的相分离潜力。结论3D基因组结构相关的生物学过程中具有IDR的蛋白质高度富集,提示相分离在细胞核结构调节中的关键作用,为蛋白质相分离和3D基因组的研究提供了完整蓝图和候选蛋白质。Objective To investigate the relationships between phase separation and 3D genome organization.Methods All the intrinsically disordered regions(IDR)were predicted by calculating the iupred2a scores of all human proteins with a minimum length of 100.Next,the main 3D-genome related biological processes regulated by phase separation were screened by gene set enrichment analysis(GSEA)which was performed according to their proportions of IDR.Results In a total 19843 human proteins,3885 proteins were rich in IDR and 1427 were involved in 3D genome organization.These IDR-enriched proteins that participated in 3D genome organization had significant preference for amino acids and modular domains.A Shiny APP named Phasepro was developed to analyze and visualize a protein′s potential of phase separation.Conclusion The biological processes involved in 3D genome organization are highly enriched with IDR,demonstrating the key potential role of phase separation in regulating nuclear structure.Our findings offer an intact blueprint and detailed protein candidates for phase separation and 3D genome research.

关 键 词：3D基因组结构 相分离 固有无序序列 生物过程 Phasepro 

分 类 号：Q78[生物学—分子生物学]