老年超重代谢综合征患者的血清代谢组学特征  

The serum metabolomic profile in elderly overweight patients with metabolic syndrome

作　　者：苏卫东[1] 方涛[2] 徐晨[1] 崔亚斌[1] 李焕明[2] Su Weidong;Fang Tao;Xu Chen;Cui Yabin;Li Huanming(Department of Clinical Laboratory,Tianjin Fourth Central Hospital,Tianjin 300140,China;Department of Cardiovascular Medicine,Tianjin Fourth Central Hospital,Tianjin 300140,China)

机构地区：[1]天津市第四中心医院检验科,天津300140 [2]天津市第四中心医院心血管内科,天津300140

出　　处：《中华老年医学杂志》2022年第5期512-516,共5页Chinese Journal of Geriatrics

基　　金：天津市卫生行业科技基金面上项目(2014KY05)。

摘　　要：目的观察老年超重代谢综合征(MS)患者和健康老年人的代谢差异特征,分析代谢物差异和相关因素。方法前瞻性研究,纳入2018年4—8月在天津市第四中心医院就诊的超重MS患者36例(MS组)和同期健康体检老年人43例(对照组),收集血清样本,采用超高效液相色谱-飞行时间质谱联用(UPLC-Q-TOF-MS)技术的非靶向代谢组学方法,分析两组入选者血清样本代谢物的差异。采用皮尔森相关统计方法分析其相关代谢指标。结果MS组与对照组比较,体质指数(26.9±2.0)kg/m^(2)比(21.7±1.4)kg/m^(2)、腰围、收缩压、空腹血糖、三酰甘油、高密度脂蛋白胆固醇比较,差异有统计学意义(均P<0.01)。血清代谢组学结果显示,老年超重MS组患者与对照组存在65种代谢差异物,这些差异物分别富集在21条通路。相关性分析结果显示,与腰围相关的差异代谢物最多,其次是体质指数,相关的差异代谢物主要为甘露糖、来苏糖、葡萄糖等单糖、亚麻酸及衍生物和焦谷氨酸等。结论老年超重MS患者与健康老年人存在多种类代谢差异物,这些代谢物与涉及超重的临床指标体质指数、腰围等相关性较高,亦包含单糖、亚麻酸衍生物和氨基酸。Objective To examine differences in metabolic characteristics and metabolites between elderly overweight patients with metabolic syndrome and healthy elderly people,and to identify related factors.Methods A group of 36 MS patients(the MS group)admitted to The Fourth Central Hospital of Tianjin from April to August 2018 and 43 elderly people(the control group)who underwent physical examination during the same period were included in this prospective study.Serum samples of the patients with metabolic syndrome and elderly healthy controls were collected,and ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)based non-targeted metabolomics was used to search for differences in metabolites between the serum samples of the two groups.The Pearson correlation statistical method was used to find related clinical factors.Results Comparison of baseline data of the enrolled participants showed that there were statistically significant differences between the two groups in body mass index[(26.9±2.0)kg/m^(2)vs.(21.7±1.4)kg/m^(2)],waist circumference,systolic blood pressure,fasting blood glucose,triglycerides and high-density lipoprotein cholesterol(P<0.01).Metabolomics results showed that there were differences in 65 serum metabolites between elderly overweight patients with metabolic syndrome and elderly normal controls,and these differences were enriched in 21 pathways.Correlation analysis showed that waist circumference had the largest number of differential metabolites,followed by body mass index.The major differential metabolites were monosaccharides such as mannose,lyxose and glucose,linolenic acid and its derivatives,and pyroglutamate.Conclusions Compared with normal elderly people,elderly patients with overweight metabolic syndrome have a variety of differential metabolites,and these metabolites are highly correlated with clinical indicators related to overweight,such as body mass index and waist circumference,and they include monosaccharides,linolenic acid derivativ

关 键 词：代谢组学 超重 代谢综合征X 

分 类 号：R589[医药卫生—内分泌]