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作 者:夏煜琪 孙宇 刘志鑫 孙瑞青 杨楠[1,2] 蒲金基 张贺[1,2,3] XIA Yuqi;SUN Yu;LIU Zhixin;SUN Ruiqing;YANG Nan;PU Jinji;ZHANG He(College of Plant Protection, College of Tropical Crops, Hainan University, Haikou 570228, China;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Haikou 571101, China;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)
机构地区:[1]海南大学植物保护学院热带作物学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室,海南海口571101 [3]中国热带农业科学院热带生物技术研究所,海南海口571101
出 处:《浙江农业学报》2022年第5期984-994,共11页Acta Agriculturae Zhejiangensis
基 金:国家重点研发计划(2019YFD1000504);中国热带农业科学院基本科研业务费(1630032022007);杧果病虫害监测与防治项目。
摘 要:BES1/BZR1是植物中特有的一类转录因子且是油菜素内酯(BR)信号转导途径的唯一转录因子,为探讨其在植物抗病抗逆反应中的作用,本研究通过运用生物信息学研究方法分析了杧果BES1s家族成员的理化性质、结构域、蛋白质结构和在不同胁迫条件下的基因表达。研究表明,杧果BES1s家族理化性质预测结果显示,成员外显子个数相差较大;结构域预测结果显示,杧果BES1s成员均具有BES1_N结构域;系统进化分析和二级结构表明,成员可分成3组,不同组之间成员存在较大差异、蛋白质三级结构预测结果显示,存在3种结构。在qRT-PCR法测定基因表达量中发现,在胶孢炭疽菌(Cg)侵染过程中MiBES1.1和MiBES1.5持续上调表达;MiBES1.12和MiBES1.13持续下调表达。在细菌性黑斑病菌(Xcm)侵染过程中,在12 h时除MiBES1.7和MiBES1.9,其他成员上调表达;在3 h时MiBES1.1~MiBES1.5和MiBES1.11下调表达。在茉莉酸甲酯(MeJA)处理过程中,MiBES1.7、MiBES1.9和MiBES1.2持续下调表达;在48 h时,除MiBES1.5和MiBES1.11~MiBES1.13其他成员下调表达。本研究结果可为杧果BES1s家族成员基因的功能研究提供依据。BES1/BZR1 is a unique transcription factor in the plant and the only transcription factor in the brassinolide(BR)signal transduction pathway.To explore the role BES1 plays on the stress resistance and diseases resistance in mango,we used bioinformatics methods to analyze the physicochemical properties,domains,protein structures and gene expression of BES1s family in mango.The results showed that in mango that the number of exons varies greatly,and all BES1s family members have the BES1_N domain.Evolution analysis and protein secondary structures showed that three groups were divided in BES1s family of mango,and there were significant differences among different groups.The qRT-PCR assay's results showed that during the infection process of Cg,MiBES1.1 and MiBES1.5 were continuously up-regulated expression,and MiBES1.12 and MiBES1.13 lasted down-regulated expression;during the infection process of Xcm,mango's BES1s family up-regulated expression at 12 h,except MiBES1.7 and MiBES1.9,and MiBES1.1~MiBES1.5 and MiBES1.11 down-regulated expression at 3 h;during the MeJA treatment process,MiBES1.7、MiBES1.9 and MiBES1.2 lasted down-regulation at 48 h,mango's BES1s gene family down-regulated expression,except MiBES1.5 and MiBES1.11~MiBES1.13.A scientific basis is provided for the further study of the BES1s family function in mango.
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