蓝桉根际菌根真菌的分离鉴定及其对蓝桉生长和光合特性的影响  

Isolation and identification of mycorrhizal fungi in rhizosphere and their effect on growth and photosynthetic characteristics of Eucalyptus globulus seedlings

作　　者：王晓丽 赵英伟 孔晓娜 曹子林[3] WANG Xiaoli;ZHAO Yingwei;KONG Xiaona;CAO Zilin(College of Forestry, Southwest Forestry University, Kunming 650224, China;State-Owned Dongmen Forest Farm in Guangxi,Fusui 532108, Guangxi, China;College of Ecology and Environment, Southwest Forestry University, Kunming 650224, China)

机构地区：[1]西南林业大学林学院,云南昆明650224 [2]广西国有东门林场,广西扶绥532108 [3]西南林业大学生态与环境学院,云南昆明650224

出　　处：《浙江农业学报》2022年第5期1015-1023,共9页Acta Agriculturae Zhejiangensis

基　　金：国家重点研发计划(2016YFD0600501)。

摘　　要：蓝桉是桉树中少有的油、材兼用树种。从云南蓝桉人工林中采集根际土,调查菌根真菌的种类和优势种,并通过控制育苗基质中菌根真菌菌群的数量,分析菌根真菌对蓝桉苗木生长和光合特性的影响,探讨影响其生长的主要菌根真菌种类,以期从菌根化育苗方面为蓝桉的壮苗培育提供指导。结果表明:从根际土中分离出3属6种菌根真菌,分别为聚丛球囊霉(Glomus aggregatum)、何氏球囊霉(Glomus hoi)、多梗球囊霉(Glomus multicaule)、摩西球囊霉(Glomus mosseae)、幼套近明球囊霉(Claroideoglomus etunicatum)、缩隔球囊霉(Septoglomus constrictum),其中,聚丛球囊霉、幼套近明球囊霉和摩西球囊霉为优势菌种(三者合计占总孢子密度的80.77%)。苗龄10个月时,随着育苗基质中菌群数量增多,蓝桉苗木的菌根侵染率增大,侵染强度增强,且接种适量的菌根真菌对苗高、地径都具有显著(P<0.05)促进作用,且能显著(P<0.05)提高苗木的叶绿素含量和表观量子效率,降低光补偿点和光饱和点。相关性分析和逐步回归分析结果显示,对苗木生长和光合能力起主要促进作用的是聚丛球囊霉、幼套近明球囊霉和缩隔球囊霉。In Eucalyptus,Eucalyptus globulus is a specific species for oil and timber dual-use.In order to provide theoretical basis and practical guidance for the cultivation of sound seedlings of E.globulus from the perspective of mycorrhizal seedlings,rhizosphere soil of E.globulus was collected from Yunnan Province.The species and dominant species of mycorrhizal fungi in rhizosphere soil were investigated.The effects of the isolated mycorrhizal fungi on the growth and photosynthetic characteristics of E.globulus seedlings were analyzed by controlling the number of mycorrhizal fungi in the nursery substrate.The main species of mycorrhizal fungi affecting seedling growth were explored through gradual regression analysis.The results showed that six species of mycorrhizal fungi were isolated from the rhizosphere soil of E.globulus plantation,which were Glomus mosseae,Glomus aggregatum,Glomus multicaule,Claroideoglomus etunicatum,Septoglomus constrictum,and Glomus hoi.The dominant mycorrhizal fungi were G.aggregatum,C.etunicatum and G.mosseae,which accumulatively accounted for 80.77%of the total spore density.At 10 months of age,the infection rate and infection intensity of mycorrhizal fungi of E.globulus seedlings were increased with the increase of the number of mycorrhizal fungi in the nursery substrate.At the ages of 5 months and 10 months,the seedling height,ground diameter of E.globulus seedlings were significantly(P<0.05)elevated with mycorrhizal fungi in the nursery substrate,the light compensation point and light saturation point of E.globulus seedlings were significantly(P<0.05)reduced,meanwhile the chlorophyll content and apparent quantum yield were significantly(P<0.05)increased.The dominant species promoting the growth and photosynthesis of E.globulus seedlings were G.aggregatum,C.etunicatum and S.constrictum,which was revealed by the correlation analysis and stepwise regression analysis.

关 键 词：蓝桉 菌根菌 分离鉴定 生长指标 光合特性 

分 类 号：S154.39[农业科学—土壤学] S792.39[农业科学—农业基础科学]