野生玫瑰多态性cpDNA和ITS引物的筛选与验证  

Screening and analysis of genome sequence difference in wild rose

作　　者：许建军 马燕[1] 吴其超 王宝盛 臧德奎[1] XU Jianjun;MA Yan;WU Qichao;WANG Baosheng;ZANG Dekui(Key Laboratory of State Forestry Administration for Silviculture of the Lower Yellow River,Forestry College, Shandong Agricultural University, Tai'an 271018, Shandong, China)

机构地区：[1]黄河下游国家林业局林业重点实验室,山东农业大学林学院,山东泰安271018

出　　处：《浙江农业学报》2022年第5期1032-1038,共7页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31870688);山东省农业良种工程(2019LZGC01802)。

摘　　要：为了筛选出具有高多态性的cpDNA和ITS序列引物,用于研究野生玫瑰自然种群的分子谱系地理,以野生玫瑰8个种群为试材,通过12对叶绿体基因组引物和4对核基因组ITS引物的PCR扩增和PCR产物测序,筛选出trnL-trnF、rpl20-rps12、rbcl、ITS1四对具有高多态性的引物。序列变异计算与系统发育树结果显示,8个野生玫瑰种群变异较低,可划分为2支。筛选出高多态性的cpDNA引物3对和ITS引物1对,适用于研究野生玫瑰系统发育和谱系地理结构,能够进一步探究濒危物种野生玫瑰的谱系地理。In order to screen out highly polymorphic cpDNA and ITS sequence primers for studying the molecular pedigree of natural populations of wild rose,8 wild rose populations were used as test materials,12 pairs of chloroplast genome primers and 4 pairs of nuclear genome ITS primers were adopted.PCR amplification and PCR product sequencing,four pairs of high polymorphic primers trnL-trnF,rpl20-rps12,rbcl,and ITS1 were screened out.Sequence variation calculation and phylogenetic tree results showed that 8 wild rose populations had low variation and could be divided into 2 branches.Three pairs of highly polymorphic cpDNA primers and 1 pair of ITS primers were screened,which were suitable for studying the phylogeny and pedigree of wild rose,and could further explore the pedigree of endangered species of wild rose.

关 键 词：野生玫瑰 ITS序列 引物筛选 系统发育树 

分 类 号：S685.12[农业科学—观赏园艺]