^(68)Ga标记的磁性铁氧体纳米颗粒PET/MRI双模态显像探针的制备及研究  

Investigation of ^(68)Ga-Labeled Magnetic Ferrite Nanoparticles PET/MRI Dual-Modal Imaging Probe

作　　者：李宛如 陈光锋 杨婷[1] 胡司琦 杨远 马晓莉 张勇[1] LI Wan-ru;CHEN Guang-feng;YANG Ting;HU Si-qi;YANG Yuan;MA Xiao-li;ZHANG Yong(Department of Nuclear Medicine,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,Chi-na;Laboratory Animal Centre of South China Agricultural University,Guangzhou 510640,China)

机构地区：[1]中山大学附属第三医院核医学科,广东广州510630 [2]华南农业大学实验动物中心,广东广州510640

出　　处：《中山大学学报（医学科学版）》2022年第3期430-436,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广州市科技计划项目(201802020033)。

摘　　要：【目的】制备^(68)Ga标记的磁性锰铁氧纳米颗粒PET/MRI双模态显像探针,考察其作为前列腺癌显像新型分子探针的可能。【方法】以超顺磁锰铁氧纳米粒子为核心,利用成纤维激活蛋白抑制剂和羟基琥珀酰亚胺-四氮杂环十二烷四乙酸(DOTA)修饰,螯合正电子放射性核素^(68)Ga,制备出纳米探针^(68)Ga-DOTA-UMFNPs-FAPI-04。对纳米探针进行基本表征,分析其细胞毒性、放射化学纯度和稳定性。观察纳米探针在正常小鼠的体内分布和对荷瘤鼠的MRI显像效果。【结果】经纯化后,^(68)Ga-DOTA-UMFNPs-FAPI-04放化纯为94%,稳定性良好。MRI T2测试显示其T2弛豫率约为39.02 mmol^(-1)·s^(-1)。正常鼠体内分布显示,^(68)Ga-DOTA-UMFNPs-FAPI-04在肝脏、脾脏的放射性摄取较高。MRI显像显示,荷瘤鼠经尾静脉注射纳米探针后30 min,肿瘤部位可观察到摄取。【结论】成功构建PET/MRI双模态显像探针^(68)Ga-DOTA-UMFNPs-FAPI-04,具有MRI T2造影剂特性,对小鼠前列腺肿瘤MRI显像良好,具备PET显像潜力。该研究为构建前列腺癌多模态成像探针提供了一个新的思路。【Objective】To prepare ^(68)Ga-labeled magnetic ferrite nanoparticles PET/MRI dual-modal imaging probe and evaluate the possibility of using it as a novel molecular probe for prostate cancer imaging.【Methods】Using superpara⁃magnetic manganese iron oxide nanoparticles as the key component,fibroblast inhibitor and DOTA-NHS ester as the modi⁃fication,along with chelating the positron radionuclide ^(68)Ga,we prepared the nanoprobe ^(68)Ga-DOTA-UMFNPs-FAPI-04.Characterization of nanoprobe and analysis of its cytotoxicity,radiochemical purity and stability were performed during the whole process.The observation of the distribution of nanoprobes in normal mice was performed.The effect of nanoprobes on MRI imaging on tumor-bearing mice was analyzed.【Results】After purification,the radiochemical purity of probe was 94%,also it had high stability.MRI T2 test showed that its T2 relaxation rate was about 39.02 mM-1·s-1.The bio-distribu⁃tion in the normal mice showed that^(68)Ga-DOTA-UMFNPs-FAPI-04 had a higher uptake in liver and spleen.MRI imag⁃ing demonstrated that tumor could be observed clearly after probe injection in 30 minutes.【Conclusions】We have success fully constructed the PET/MRI dual-modal imaging probe^(68)Ga-DOTA-UMFNPs-FAPI-04.It has the characteristics of MRI T2 contrast agent and it also performs well in the MRI imaging of prostate tumors and has the potential of PET imag⁃ing.The study provides a new idea for constructing a multi-modal imaging probe for prostate cancer.

关 键 词：PET/MRI双模态成像 磁性纳米颗粒 成纤维激活蛋白抑制剂 

分 类 号：R817.1[医药卫生—影像医学与核医学]