朱砂七碱制前后的化学成分分析  

Analysis of chemical composition of Polygonum cillinerve(Nakai)Ohwi before and after alkali processing

作　　者：程茜菲[1] 张玩涛 韩小娟 宋小妹[2] CHENG Qianfei;ZHANG Wantao;HAN Xiaojuan;SONG Xiaomei(Shaanxi Institute of International Trade and Commerce,Xi'an 712046,China;Shaanxi University of Traditional Chinese Medicine,Xi'an 712046,China)

机构地区：[1]陕西国际商贸学院,西安712046 [2]陕西中医药大学,西安712046

出　　处：《西北药学杂志》2022年第3期8-11,共4页Northwest Pharmaceutical Journal

基　　金：陕西省中药绿色制造技术协同创新中心平台项目(编号:2019XT-3-04);陕西省中医药管理局科研项目(编号:2021-ZZ-ZY005);陕西省科技厅重点研发计划项目(编号:2022SF-355)。

摘　　要：目的以朱砂七生品和碱制品为研究对象、化学成分为依据,分别从药材整体和单个化学成分两方面进行质量分析。方法采用高效液相色谱法,色谱柱为C_(18),柱温为35℃,采用甲醇-10mL·L^(-1)磷酸水溶液进行梯度洗脱,检测波长为254 nm,建立朱砂七生品、碱制品指纹图谱并测定大黄素的含量,用统计分析软件和2012版“中药色谱指纹图谱相似度评价系统”进行数据和图谱分析。结果朱砂七碱制前、后指纹图谱相似性降低,大黄素含量有所增加。结论可初步阐明碱制朱砂七药效物质基础,为朱砂七的深入研究奠定基础。Objective This paper took the raw and alkali processing product of Polygonum cillinerve(Nakai)Ohwi(Zhushaqi)as the research objects.The quality evaluation was carried out from the overall composition and the single component of Zhushaqi.Methods The fingerprint spectrum of the raw and the alkali processing Zhushaqi and the content determination of emodin were carried out by high performance liquid chromatography(HPLC)at 254 nm.The separation was performed on a C_(18) column(35℃)with gradient elution.The mobile phase consisted of methanol and 10 mL•L^(-1) phosphoric acid.The fingerprinting analysis were carried out by using statistical analysis software and Similarity Evaluation System for Chromatographic Fingerprint of TCM(version 2012).Results The similarity of fingerprints spectra of Zhushaqi before and after alkali processing was reduced while the content of emodin was increased.Conclusion The study preliminarily clarifies the pharmacodynamic substance basis of alkaline Zhushaqi,which provides scientific basis for its further development research and clinical application.

关 键 词：朱砂七 碱制 大黄素 指纹图谱 

分 类 号：R284[医药卫生—中药学]