基于HPLC-MS与Cocktail探针药物法研究五味子提取物对大鼠CYP450酶的影响  

Study on the effect of Schisandra chinensis extract on CYP450 enzyme in rats based on HPLC-MS and cocktail probe drug method

作　　者：李莉 童黄锦[2] 汪丽君[3] 苏联麟 陆兔林[3] 李昱 LI Li;TONG Huangjin;WANG Lijun;SU Lianlin;LU Tulin;LI Yu(Bozhou Food and Drug Inspection Center,Bozhou 236800,China;Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]亳州市食品药品检验中心,亳州236800 [2]南京中医药大学附属中西医结合医院,南京210028 [3]南京中医药大学药学院,南京210023

出　　处：《西北药学杂志》2022年第3期61-70,共10页Northwest Pharmaceutical Journal

基　　金：江苏省研究生科研创新计划项目(编号:KYCX20_1505);南京药学会-常州四药医院药学科研基金项目(编号:2018YX006)。

摘　　要：目的研究五味子提取物对大鼠肝脏中细胞色素P450酶(CYP450酶)的影响。方法采用高效液相色谱串联质谱(high performance liquid chromatography tandem mass spectrometry,HPLC-MS)分析技术建立稳定的探针底物色谱分析法,结合Cocktail分析单次给予五味子提取物对大鼠体内CYP2D6、CYP2C19、CYP2C8、CYP2A6、CYP2B6、CYP2C196种CYP450亚型酶活性的影响。结果五味子提取物对大鼠肝脏中CYP2D6、CYP2C19和CYP2C8的活性具有显著的抑制作用,而对CYP2A6、CYP2B6和CYP2C19的活性具有显著的诱导作用(P<0.05)。结论用HPLC-MS结合Cocktail探针药物法研究五味子提取物对大鼠CYP450酶影响的准确性及可靠性较强,五味子对CYP450酶多个亚型的不同调控作用可能是其发挥降酶保肝作用的重要机制。Objective To study the effect of Schisandra chinensis extract on 6 common and important CYP450 enzymes in rat liver.Methods HPLC-MS was used to establish a stable probe substrate chromatography method, and cocktail analysis was used to evaluate the activities of 6 CYP450 isoforms CYP2 D6,CYP2 C19,Cyp2 C8,CYP2 A6,CYP2 B6 and CYP2 C19 in rats.Results Schisandra chinensis extract could significantly inhibit the activities of CYP2 D6,CYP2 C19 and CYP2 C8,and induce the activities of CYP2 A6,CYP2 B6 and CYP2 C19(P<0.05).Conclusion The method established in this study is reliable and accurate.The different regulatory effects of Schisandra chinensis on CYP450 isoforms may be one of the important mechanisms of Schisandra chinensis on reducing enzyme and protecting liver.

关 键 词：五味子 高效液相色谱串联质谱(HPLC-MS) COCKTAIL 探针药物 CYP450酶 

分 类 号：R965[医药卫生—药理学]