石墨烯/聚乙烯亚胺复合修饰电极伏安测定鸢尾苷  

Voltammetric determination of tectoridin by using graphene/polyethyleneimine modified glassy carbon electrode

作　　者：王安亭 卫应亮 王芳 李永程 WANG An-ting;WEI Ying-liang;WANG Fang;Ll Yong-cheng(School of Environmental Engineering and Chemistry,Luoyang Institute of Science and Technology,Luoyang 471023,China;Luoyang Beiyuan Biotechnology Limited Company,Luoyang 471000,China)

机构地区：[1]洛阳理工学院环境工程与化学学院,河南洛阳471023 [2]洛阳北苑生物科技有限公司,河南洛阳471000

出　　处：《化学研究与应用》2022年第5期988-994,共7页Chemical Research and Application

基　　金：国家自然科学基金项目(21968031)资助;河南省科技攻关项目(182102311037)资助;河南省高等学校青年骨干教师资助计划(2009GGJS-123)资助。

摘　　要：制备了石墨烯/聚乙烯亚胺(GR/PEI)复合物,采用紫外-可见吸收光谱、电化学交流阻抗方法对复合物进行表征,由此制备了石墨烯/聚乙烯亚胺复合修饰电极(GR/PEI/GCE)。采用循环伏安法研究了支持电解质、pH、扫描速度等对鸢尾苷电化学行为的影响。同时,调查了GR/PEI/GCE复合修饰电极的重现性、稳定性,不同物质对测定鸢尾苷的干扰。在5.0×10^(-7)~1.0×10^(-5) mol·L^(-1)浓度范围,鸢尾苷氧化峰(P_(3))的峰电流与其浓度呈良好的线性关系,线性相关系数为0.999,检出限为1.67×10^(-7) mol·L^(-1)。加标回收结果显示:鸢尾苷的回收率在94.0%~105.0%左右,表明该方法测量的准确度较高。采用高效液相色谱法(HPLC)进行了测量对比,发现该方法与HPLC两种方法测定结果一致。Graphene/polyethyleneimine(GR/PEI)composites were prepared,and characterized by ultraviolet-visible absorption spectroscopy,AC impedance spectroscopy.Based on this composites,graphene/polyethyleneimine composite modified electrode(GR/PEI/GCE)was developed.The effect of measurement conditions,such as supporting electrolyte,pH,scanning speed on the electrochemical behaviors of tectoridin(TEC)was investigated by cyclic voltammetry.Simultaneously,the reproducibility and stability of GR/PEI/GCE were studied.The interference of some foreign species on the determination of tectoridin was analyzed.In the concentration range of 5.0×10^(-7)~1.0×10^(-5) mol·L^(-1),the peak(P_(3))current of tectoridin has a good linear relationship with its concentration,and the detection limit is 1.67×10^(-7) mol·L^(-1).The recovery of tectoridin was 94.0~105.0%,which indicated that the accuracy of the proposed method was reliable.The measurement result of tectoridin by the electrochemical method was consistent with that of high-performance liquid chromatography(HPLC).

关 键 词：鸢尾苷 石墨烯/聚乙烯亚胺复合修饰电极 伏安法 电化学行为 

分 类 号：O657.15[理学—分析化学]