一种多羧酸配体金属离子荧光探针的光谱性能与识别机制研究  

Spectral Properties and Recognition Mechanism of A Polycarboxyl-receptor Fluorescent Probe for Metal Ions

作　　者：成昭[1] 郑蕾[1] 何昊[1] 徐玥[1] CHENG Zhao;ZHENG Lei;HE Hao;XU Yue(School of Pharmacy,Xi’an Medical University,Xi'an 710021,China)

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《精细化工中间体》2022年第2期13-19,68,共8页Fine Chemical Intermediates

基　　金：陕西省教育厅自然科学项目(20JK0902);陕西省科技厅项目(2022JM-542);教育部“春蕾计划”项目(2020-14);陕西省科技厅项目(2019JW-073)。

摘　　要：基于ICT机制构筑探针结构,定向连接识别基团多羧酸配体与荧光基团苯并咪唑,合成得到探针T14,并将其用于水系金属离子的识别实验。结果显示,探针对Zn^(2+)、Cu^(2+)、Hg^(2+)表现特征响应、荧光性能稳定,探针与目标离子以1∶1定量结合、线性关系良好,能够进行水体系中Zn^(2+)、Cu^(2+)、Hg^(2+)的定性检测和定量分析,初步满足μmol/L数量级的生理Zn^(2+)、Cu^(2+)、Hg^(2+)浓度检测。此外,结合荧光、紫外等光谱学手段,初步阐明了探针对Cu^(2+)、Hg^(2+)和Zn^(2+)的不同识别机制。Based on intramolecular charge transfer(ICT),fluorescent probeT14was synthesized through incorporating benzimidazole as the fluorophore and polycarboxylic receptor.This obtainedT14was applied in the recognition of aqueous metal ions.Experimental results indicated thatT14was a Zn^(2+)/Cu^(2+)/Hg^(2+)-detecting probe with characteristic and steady responses in fluorescence intensity,accompanied with good linear relationships and1∶1 complexation stoichiometry for the combinations ofT14-Zn^(2+)/Cu^(2+)/Hg^(2+).Besides the qualitative and quantitative analyses of aqueous Zn^(2+)/Cu^(2+)/Hg^(2+)in a concentration level of μmol/L,the different recognition mechanism of probeT14towards Zn^(2+)/Cu^(2+)/Hg^(2+)was illustrated by UV-Vis and fluorescence spectra.

关 键 词：荧光探针 多羧酸配体 即时响应 定量分析 

分 类 号：O622.6[理学—有机化学]