电针联合骨髓间充质干细胞治疗化疗致大鼠卵巢功能不全  

Electroacupuncture combined with bone marrow mesenchymal stem cells in the treatment of chemotherapy-induced premature ovarian insufficiency in rats

作　　者：马牧南 解军 桑宇超 黄磊 张国栋 杨晓丽[3] 傅松涛 Ma Munan;Xie Jun;Sang Yuchao;Huang Lei;Zhang Guodong;Yang Xiaoli;Fu Songtao(Department of Biochemistry and Molecular Biology,School of Basic Medicine,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China;Shanxi Provincial Biomedical Health Graduate Education Innovation Center,Taiyuan 030001,Shanxi Province,China;Reproductive Center,Taiyuan Central Hospital,Taiyuan 030001,Shanxi Province,China;Shanxi Binda Stem Cell Technology Co.,Ltd.,Taiyuan 030001,Shanxi Province,China)

机构地区：[1]山西医科大学基础医学院生物化学与分子生物学教研室,山西省太原市030001 [2]山西省生物医药健康研究生教育创新中心,山西省太原市030001 [3]太原市中心医院生殖中心,山西省太原市030001 [4]山西宾大干细胞科技有限公司,山西省太原市030001

出　　处：《中国组织工程研究》2023年第1期1-7,共7页Chinese Journal of Tissue Engineering Research

基　　金：山西省重点学科建设基金,项目负责人:解军;山西省‘1331工程’重点学科项目建设基金,项目负责人:解军。

摘　　要：背景:骨髓间充质干细胞治疗卵巢功能不全有不错的效果,但仍存在归巢效率低、效果欠佳等问题。目的:探讨电针疗法联合骨髓间充质干细胞治疗化疗型卵巢功能不全的效果。方法:从32只SD大鼠中随机选择8只做正常组,其余随机分为卵巢功能不全组、干细胞组、联合组,每组8只,采用腹腔注射环磷酰胺溶液制备卵巢功能不全动物模型。造模结束后,正常组不做处理,卵巢功能不全组尾静脉注射1 mL生理盐水,干细胞组与联合组尾静脉注射1 mL骨髓间充质干细胞悬液(细胞数为2×106),联合组大鼠进行电针治疗,30 min/d,持续7 d,治疗结束后取心脏血与卵巢组织观察疗效。结果与结论:①与卵巢功能不全组相比,干细胞组及联合组大鼠血清雌二醇激素水平明显升高,卵泡刺激素水平明显降低;②与卵巢功能不全组相比,干细胞组及联合组大鼠卵巢卵母细胞数量明显增加;③与干细胞组相比,联合组的骨髓间充质干细胞归巢水平明显增加(P=0.0043),CXCR4蛋白表达水平升高(P=0.036);④与卵巢功能不全组相比,干细胞组及联合组大鼠卵巢凋亡细胞明显减少,Bax蛋白表达水平降低,Bcl-2蛋白表达水平升高;与干细胞组相比,联合组Bax表达水平明显降低(P=0.0284),Bcl-2表达水平明显升高(P=0.0364);⑤结果显示,电针能促进骨髓间充质干细胞归巢,并增强骨髓间充质干细胞的抗凋亡作用。BACKGROUND:Bone marrow mesenchymal stem cells have good effects on treating premature ovarian insufficiency.However,there are still problems such as low homing efficiency and poor effects.OBJECTIVE:To observe the effect of electroacupuncture combined with bone marrow mesenchymal stem cells in treatment of chemotherapy-induced premature ovarian insufficiency.METHODS:Eight SD rats were randomly selected as the normal group from 32 SD rats,and others were randomly divided into ovarian insufficiency group,stem cell group,and combined treatment group,with 8 rats in each group.Cyclophosphamide solution was intraperitoneally injected to create premature ovarian insufficiency model.After modeling,the normal group was left untreated,and the ovarian insufficiency group was injected with 1 m L normal saline via tail vein.The stem cell group and the combined treatment group were injected with 2×106 bone marrow mesenchymal stem cell suspension 1 m L through the tail vein.Rats in the combined treatment group were treated with electroacupuncture for 30 minutes per day for 7 day.After the treatment,heart blood and ovarian tissue were taken to observe the curative effects.RESULTS AND CONCLUSION:(1) Compared with the ovarian insufficiency group,the levels of serum estradiol in the stem cell group and the combined treatment group were significantly increased,and the levels of follicle stimulating hormone were significantly reduced.(2) Compared with the ovarian insufficiency group,the number of ovarian oocytes in the stem cell group and the combined treatment group increased significantly.(3) Compared with the stem cell group,the homing level of bone marrow mesenchymal stem cells in the combined treatment group increased significantly(P=0.004 3),and the expression level of CXCR4 protein also increased(P=0.036).(4) Compared with the ovarian insufficiency group,the two groups receiving treatment had fewer apoptotic cells,lower Bax protein expression levels,and higher Bcl-2 protein expression levels in ovary.Compared with the stem cel

关 键 词：电针 骨髓间充质干细胞 大鼠 化疗型卵巢功能不全 卵母细胞 归巢 凋亡 趋化因子受体 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]