磁场对间充质干细胞成骨的生物学效应  

Biological effects of magnetic field on osteogenesis of mesenchymal stem cells

作　　者：张璟岚 张滨婧 陈艺菲 张辰玥 胡芝爱 胡海琨[1] Zhang Jinglan;Zhang Binjing;Chen Yifei;Zhang Chenyue;Hu Zhiai;Hu Haikun(State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院,四川省成都市610041

出　　处：《中国组织工程研究》2023年第1期145-151,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(82001108),项目负责人:胡芝爱;中国博士后科学基金(2020M683331),项目负责人:胡芝爱;四川大学华西口腔医学院(华西口腔医院)科研经费资助项目(RCDWJS2022-4),项目负责人:胡芝爱。

摘　　要：背景:间充质干细胞具有自我复制和多向分化潜能,在骨组织再生领域备受关注。磁场作为非侵入性的物理刺激因素能够有效调控间充质干细胞的黏附、增殖、成骨向分化和矿化等生物学行为,在骨组织再生医学领域具有广泛的应用前景。磁场的效应取决于磁场强度、频率和暴露时间等参数。由于窗口效应,磁场促进骨再生的适宜参数范围仍有待明确。目的:回顾不同类型磁场和不同磁场参数对间充质干细胞成骨分化的影响及可能机制,并归纳总结促增殖和成骨向分化的适宜参数范围。方法:以“magnetic field,static magnetic field,pulsed electromagnetic field,mesenchymal stem cells,proliferation,osteogenic differentiation,bone regeneration,bone repair”为英文检索词,以“磁场,静磁场,脉冲电磁场,间充质干细胞,增殖,成骨分化,骨再生”为中文检索词,分别检索PubMed数据库和中国知网,主要检索时间范围为2010年1月至2021年9月,同时纳入少量远期经典文献。通过文题及摘要进行初筛,排除重复性、低相关性和低价值的研究,最终纳入56篇文献进行综述。结果与结论:(1)中等强度静磁场、低频或低强度脉冲电磁场促进间充质干细胞增殖和成骨向分化的效果良好,而高频脉冲电磁场产生明显的抑制作用;(2)磁场调控效果与总暴露时间呈正相关,但其效应并不随单次间歇暴露的时间延长而增加;(3)干细胞类型、分化阶段和细胞密度等磁场外因素对磁场的生物学效应也具有复杂的影响,临床上应给予关注;(4)磁场对质膜、细胞骨架和离子通道等细胞靶点产生作用,作用机制主要包括磁机械相互作用、电动力相互作用和自由基对效应。BACKGROUND:Mesenchymal stem cells,with self-replication and multidirectional differentiation potential,attracted much attention in the field of bone tissue regeneration.As a non-invasive physical stimulation factor,the magnetic field can effectively regulate the biological behaviors of mesenchymal stem cells such as adhesion,proliferation,osteogenic differentiation and mineralization,which means that it has a broad application prospect in bone tissue regeneration medicine.The effect of the magnetic field depends on parameters such as magnetic field strength,frequency and exposure time.Due to the window effect,the appropriate parameter range of magnetic field promoting bone regeneration remains to be clarified.OBJECTIVE:To review the effects of different magnetic fields and different magnetic parameters on osteogenic differentiation of mesenchymal stem cells as well as the possible mechanisms,and to summarize the appropriate parameters for promoting proliferation and osteogenic differentiation.METHODS:Databases of CNKI and PubMed were searched for relevant articles published from January 2010 to September 2021 using the keywords including“magnetic field,static magnetic field,pulsed electromagnetic field,mesenchymal stem cells,proliferation,osteogenic differentiation,bone regeneration,bone repair”in Chinese and English.A few classic early articles were also included.By reading the titles and abstracts,a preliminary screening was conducted.Repetitive studies,low-quality or irrelevant articles were excluded.Finally,a total of 56 articles were retained and analyzed for this review.RESULTS AND CONCLUSION:(1)Moderate static magnetic field,low frequency or low intensity pulsed electromagnetic field,have positive effects on the proliferation and osteogenic differentiation of mesenchymal stem cells.High-frequency pulsed electromagnetic field produces obvious inhibitory effect.(2)The magnetic field modulation effect was positively correlated with the total exposure time,but its effect did not increase with the time of s

关 键 词：磁场 间充质干细胞 静磁场 脉冲电磁场 磁场参数 增殖 成骨分化 骨再生 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]