微丝电极阵列的参考电极内置与外置的对比研究  

A comparative study of microwire electrode array with built-in and external reference electrodes

作　　者：张利娜 杜相欣 张雨彤 郭霞[1] 郝娜 赵欣[1] 张宇[1] ZHANG Li-na;DU Xiang-xin;ZHANG Yu-tong;GUO Xia;HAO Na;ZHAO Xin;ZHANG Yu(Key Laboratory of Cell Physiology,Ministry of Education,Department of Physiology,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学生理学系细胞生理学教育部重点实验室,太原030001

出　　处：《中国应用生理学杂志》2022年第1期85-90,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金项目(81371254);山西省‘1331工程’重点学科建设计划经费(XK201708);山西省自然科学基金项目(201801D121318)。

摘　　要：目的:通过对比内置和外置参考电极的微丝电极阵列在记录大鼠脑神经元放电过程中的优缺点,优化微丝电极阵列的制作与埋置,为多通道电生理实时记录系统提供更加实惠、优异的媒介工具。方法:采用镍铬合金丝、电路板、电极引脚和地线(银线)制作16通道的微丝电极阵列,通过内置(参考电极与电极阵列并列排布)或外置(参考电极与地线分别焊接在电极一侧的两端)微丝电极阵列的参考电极,观察对比两种电极在记录大鼠ACC脑区神经元放电中的区别。实验大鼠分为内置组(8只)和外置组(9只),检测指标有信噪比(n=8)、放电幅度(n=380)和放电频率(n=54)。结果:内置与外置参考电极的微丝电极阵列均可顺利记录出大鼠ACC脑区神经元的电信号;与外置组相比,内置组的神经元电信号具有信噪比高(P<0.05)、背景信号幅度小、受噪音干扰小,和放电幅度大(P<0.05)的优点;锋电位放电频率没有显著差异(P>0.05)。结论:在记录大鼠ACC脑区神经元电活动时,内置参考电极的微丝电极阵列记录到更高信噪比、更大放电幅度的电信号,为多通道电生理技术提供更加可靠的工具。Objective:To compare the difference between the built-in and external reference electrode of microwire electrode array in the process of recording rat brain neuron firings,optimizing the production and embedding of the microwire electrode array,and providing a more affordable and excellent media tool for multi-channel electrophysiological real-time recording system.Methods:A 16 channel microwire electrode array was made by using nickel chromium alloy wires,circuit board,electrode pin and ground wires(silver wires).The reference electrode of the microwire electrode array was built-in(the reference electrode and electrode array were arranged in parallel)or external(the reference electrode and ground wire were welded at both ends of one side of the electrode),and the difference between the two electrodes was observed and compared in recording neuronal discharges in ACC brain area of rats.Experimental rats were divided into built-in group and external group,n=8-9.The test indicators included signal-to-noise ratio(n=8),discharge amplitude(n=380)and discharge frequency(n=54).Results:The microwire electrode array with both built-in and external reference electrodes successfully recorded the electrical signals of neurons in the ACC brain region of rats.Compared with the external group,the electrical signals of neurons in built-in group had the advantages of a higher signal-to-noise ratio(P<0.05),a smaller amplitude of background signals and less noise interference,and a larger discharge amplitude(P<0.05);there was no significant difference in spike discharge frequency recorded by these two types of electrodes(P>0.05).Conclusion:When recording the electrical activity of neurons in the ACC brain region of rats,the microwire electrode array with built-in reference electrode recorded electrical signals with higher signal-to-noise ratio and larger discharge amplitude,providing a more reliable tool for multi-channel electrophysiology technology.

关 键 词：多通道电生理 微丝电极阵列 参考电极 信噪比 脑机接口 大鼠 

分 类 号：R338.8[医药卫生—人体生理学]