伊维菌素合成产物中杂质的分离与鉴定  

Separation and Identification of Impurities in Synthetic Products of Ivermectin

作　　者：高月麒 王克华 马志珺[1] 林旸[1] 李晓露[1] 任风芝[1] GAO Yueqi;WANG Kehua;MA Zhijun;LIN Yang;LI Xiaolu;REN Fengzhi(NCPC New Drug Research & Development Co.,Ltd.,National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,Shijiazhuang 052165,China;NCPC Aino Co.,Ltd.,Shijiazhuang 052165,China)

机构地区：[1]华北制药集团新药研究开发有限责任公司,微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,河北石家庄052165 [2]华北制药集团爱诺有限公司,河北石家庄052165

出　　处：《化学与生物工程》2022年第5期26-31,共6页Chemistry & Bioengineering

基　　金：河北省科技研发平台建设专项(199676154H)。

摘　　要：以阿维菌素为起始原料,经催化加氢反应得到伊维菌素粗品。采用二维色谱纯化技术对伊维菌素粗品中的关键杂质进行分离提纯,鉴定了4个杂质,其中杂质1为阿维菌素B_(1a),杂质2为3′-OH-H_(2)B_(1a),杂质3为EP9.0中有关物质H,杂质4为EP9.0中有关物质K(H_(4)B_(1a)异构体)。Using Avermectin as a starting material,we obtained crude Ivermectin by catalytic hydrogenation.Moreover,we separated and purified the key impurities in crude Ivermectin by two-dimensional chromatographic purification technology.Four impurities are identified,among which,impurity 1 is identified as Avermectin B_(1a),impurity 2 is identified as 3′-OH-H_(2)B_(1a),impurity 3 is identified as related substance H in EP9.0,and impurity 4 is identified as related substance K(H_(4)B_(1a) isomer)in EP9.0.

关 键 词：伊维菌素 合成 杂质 色谱纯化 结构鉴定 

分 类 号：TQ465.5[化学工程—制药化工]