白桦酯醇对白桦悬浮细胞生长生理的影响  

Effects of Betulin on Suspension Cell Growth Physiology of Betula platyphylla

作　　者：王勃 姜洋 詹亚光[1] 范桂枝[1] WANG Bo;JIANG Yang;ZHAN Ya-guang(College of Life Sciences,Northeast Forestry University,Harbin,Heilongjiang 150040)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2022年第10期1-5,共5页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省自然科学基金项目(C2016005);国家自然科学基金面上项目(32171829)。

摘　　要：以白桦悬浮细胞为试材,将0.1~5.0μmol/L白桦酯醇添加到培养7 d的白桦细胞悬浮培养体系中处理12 h~14 d,采用比色法分析白桦悬浮细胞活力、丙二醛和NO含量等,解析白桦酯醇对白桦悬浮细胞生长生理的影响。结果表明,不同浓度白桦酯醇处理12 h~14 d,白桦悬浮细胞鲜重、活力、pH和电导率与同期对照相比无显著差异,其最大值分别在处理后14 d、7 d、14 d和12 h;与同期对照相比,白桦酯醇处理后白桦悬浮细胞中MDA含量、超氧化物歧化酶活性和过氧化物酶活性及其基因表达水平基本呈增加趋势,在处理14 d时达最大值;除1.0和5.0μmol/L白桦酯醇处理显著增加了白桦悬浮细胞中NO含量外,其他处理下NO和亚硝基硫醇含量与对照相比未产生显著影响。可见0.1~5.0μmol/L白桦酯醇对白桦悬浮细胞生长未产生显著影响。With birch suspension cells used as testing materials,betulin of 0.1-5.0μmol/L was added into the culture system of suspension cells of Betula platyphylla for culturing 7 d to the treatment which lasted 12 h-14 d.Colorimetric method was applied to analyzing the cell viability,malondialdehyde and NO content of the suspension cells of Betula platyphylla,etc.,which helped to dissect and analyze the effects of betulin on the growth and physiology of suspension cells of Betula platyphylla.The results showed that after treatment with different concentrations of betulin for 12 h to 14 d,there was no significant difference in fresh weight,viability,pH and electrical conductivity of the suspension cells of Betula platyphylla compared with the control in the same period,and the maximum values were at 14 d,7 d,14 d and 12 h after treatment,respectively.Compared with the control in the same period,the MDA content,superoxide dismutase activity,peroxidase activity and gene expression levels in the suspension cells of Betula platyphylla after betulin alcohol treatment basically showed an increasing trend,and reached the maximum value at 14 days of treatment.Except for the treatment of 1.0 and 5.0μmol/L betulin significantly increased the NO content in the suspension cells of Betula platyphylla,the content of NO and nitrosothiol under other treatments had no significant effect compared with the control.It could be seen that 0.1-5.0μmol/L betulin had no significant effect on the growth of the suspension cells of Betula platyphylla.

关 键 词：白桦酯醇 白桦悬浮细胞 细胞活性 生长生理 

分 类 号：S718[农业科学—林学]