怀牛膝可培养内生真菌的多样性研究  

Study on Diversity of Culturable Endophytic Fungi from Achyranthes bidentata Blume

作　　者：孙思胜[1] 张晓娟 马传贵 刘洋洋 陈林[5] 张岗 SUN Si-sheng;ZHANG Xiao-juan;MA Chuan-gui(College of Food and Pharmacy,Xuchang University/Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety of Henan Province,Xuchang,Henan 461000;Quartermaster Engineering Technology Institute,Academy of Military Science of the CPLA,Beijing 100010;Beijing Jingcheng Biological Technology Co.,Ltd.,Beijing 102600)

机构地区：[1]许昌学院食品与药学院/河南省食品安全生物标识快检技术重点实验室,河南许昌461000 [2]军事科学院军需工程技术研究所,北京100010 [3]北京京诚生物科技有限公司,北京102600 [4]中国医学科学院药用植物研究所海南分所,海南海口570216 [5]黄河科技学院,郑州市天然产物合成生物学重点实验室,河南省小分子新药研发国际联合实验室,河南郑州450063 [6]陕西中医药大学药学院/陕西省秦岭中草药应用开发工程技术研究中心,陕西咸阳712046

出　　处：《安徽农业科学》2022年第10期149-151,156,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省科技攻关项目(192102310441,202102110055);陕西省高校青年杰出人才支持计划。

摘　　要：[目的]探讨道地药材怀牛膝(Achyranthes bidentata Blume)内生真菌的多样性组成及定殖情况。[方法]通过组织块分离法,选取二年生怀牛膝茎部和根部作为分离对象,进行内生真菌分离,并采用形态学和分子生物学2种方法对分离得到的真菌进行鉴定。[结果]从怀牛膝材料中共分离纯化获得136株内生真菌,其中根部分离得到86株,茎部分离得到50株,根据形态和分子生物学特征,它们属于8目11科11属;其中,怀牛膝根部内生真菌定殖率较高(65.0%),茎部内生真菌定殖率较低(37.5%)。茎部分离的内生真菌只有8种,根部分离得到11种。[结论]怀牛膝根部内生真菌表现出了较高的种属多样性。在怀牛膝根部分离的内生真菌中,优势菌属是镰孢属和Moesziomyces。[Objective] To explore the diversity composition and colonization of endophytic fungi in Achyranthes bidentata Blume.[Method]By the method of tissue block separation, the stems and roots of biennial Achyranthes bidentata were selected as the separation objects, and the endophytic fungi were isolated, and the isolated fungi were identified by morphological and molecular biology methods.[Result]A total of 136 endophytic fungi were isolated and purified from Achyranthes bidentata materials, of which 86 strains were isolated from roots and 50 strains from stems.According to morphological and molecular biological characteristics, they belonged to 8 orders, 11 families and 11 genera.Among them, the colonization rate of endophytic fungi in the root of Achyranthes bidentata was higher(65.0%),and the colonization rate of endophytic fungi in the stem was lower(37.5%).Only 8 species of endophytic fungi were isolated from the stem, and 11 species were isolated from the root.[Conclusion]The endophytic fungi in the root of Achyranthes bidentata shows high species diversity.Among the endophytic fungi isolated from the roots of Achyranthes bidentata, dominant genera were Fusarium and Moesziomyces.

关 键 词：怀牛膝 内生真菌 根部 茎部 多样性 

分 类 号：S567.239[农业科学—中草药栽培]