垃圾渗滤液膜浓缩液中总氮深度脱除的工艺优化及微生物机理解析  

Deep removal efficiency of total nitrogen from landfill leachate membrane concentrate:Process optimization and microbial mechanism analysis

作　　者：刘宁[1,2] 陆杰 罗琦 邱晨晨 杨庆 黄开龙 叶林[2] 张徐祥[2] LIU Ning;LU Jie;LUO Qi;QIU Chenchen;YANG Qing;HUANG Kailong;YE Lin;ZHANG Xuxiang(Nanjing University Jinling College,Nanjing 210032,China;State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210023,China;Nanjing Jiangdao Institute of Environmental Research Co.,Ltd.,Nanjing 210019,China)

机构地区：[1]南京大学金陵学院,江苏南京210032 [2]南京大学环境学院,污染控制与资源化研究国家重点实验室,江苏南京210023 [3]南京江岛环境科技研究院有限公司,江苏南京210019

出　　处：《工业水处理》2022年第5期67-76,共10页Industrial Water Treatment

基　　金：国家自然科学基金项目(52100082);江苏省重点研发计划(社会发展)项目(BE2019635);污染控制与资源化研究国家重点实验室开放基金项目(PCRRF19004,PCRRF20018);江苏高校青蓝工程中青年学术带头人(苏教师[2017]15号);2020年度南京大学金陵学院校企共建课题“水处理工程设计”(0010522014)。

摘　　要：为了开发高效低耗的垃圾渗滤液膜浓缩液处理方法,以江苏某垃圾焚烧厂渗滤液膜浓缩液为研究对象,通过优化调控碳源种类、水力停留时间(HRT)、碳氮比(C/N)等运行条件,考察反应器的脱氮效能,并揭示其深度脱氮的微生物学机理。研究表明,从碳源种类来看,葡萄糖体系和乙酸钠体系的反硝化效能相较甲醇体系更加高效稳定、耐冲击负荷,且葡萄糖体系处理成本为乙酸钠体系的一半;从HRT、C/N来看,工艺运行过程调控HRT、C/N参数对系统脱氮效能造成的冲击均是可逆的。通过优化获得的最佳运行条件为以葡萄糖为外加碳源,HRT=12 h,C/N=3.5。基于16S rRNA高通量测序结果显示碳源种类对反应器中微生物群落结构影响较大,而HRT和C/N对脱氮微生物群落结构影响较小。稳定运行期间主要优势反硝化脱氮菌为Vibrio(弧菌属)、Marinobacter(海杆菌属)、Halomonas(盐单胞菌属)、Paracoccus(副球菌属)、Rhodocyclaceae(红环菌科)、Carnobacteriaceae(肉杆菌科)、Porphyromonadaceae(紫单胞菌科)、Pseudomonas(假单胞菌属)、Xanthomonadaceae(黄单胞菌科)和Flavobacteriaceae(黄杆菌科)。In order to explore a more efficient and low consumption landfill leachate membrane concentrate(LLC)treatment method,the LLC of a waste incineration plant in Jiangsu was chosen as the research object.This research investigated the optimization of UASB denitrification process and evaluated the effects of different operating conditions(carbon source type,hydraulic retention time and carbon nitrogen ratio)on denitrification efficiency,microbial community structure and treatment cost.The results showed that the denitrification efficiency of glucose system and sodium acetate system was more efficient,stable and impact resistant than that of methanol system,and the treatment cost of glucose system was half of that of sodium acetate system.In terms of HRT and C/N,the effects of HRT and C/N on the efficiency of denitrification system were reversible.The optimum operating parameters were as follows:the carbon source,HRT,C/N were glucose,12 h,and 3.5,respectively.Furthermore,16S rRNA highthroughput sequencing technology was used to investigate the effects of operating conditions on the microbial com-munity structure of UASB.The results showed that carbon source types had a greater influence on the microbial com-munity structure in UASB,while HRT and C/N had a smaller influence on the bacterial structure composition.The main dominant denitrifying bacteria were Vibrio,Marinobacter,Halomonas,Paracoccus,Rhodocyclaceae,Carnobacteriaceae,Porphyromonadaceae,Pseudomonas,Xanthomonadaceae and Flavobacteriaceae.

关 键 词：垃圾渗滤液膜浓缩液 碳源种类 水力停留时间 碳氮比 成本分析 

分 类 号：X703.1[环境科学与工程—环境工程]