1型糖尿病食蟹猴左心室心肌组织超微结构变化  

Ultrastructural changes in left ventricular myocardium of type 1 diabetic mellitus cynomolgus monkeys

作　　者：滕夏虹[1] 陈帅[2,3,4] 邹春林 杜可晨[2,3,4] 曹津津 宋琼 Teng Xiahong;Chen Shuai;Zou Chunlin;Du Kechen;Cao Jinjin;Song Qiong(School of International Education,Guangxi Medical University,Nanning 530021;Center for Translational Medicine,Guangxi Medical University,Nanning 530021;School of Preclinical Medicine,Guangxi Medical University,Nanning 530021;Key Laboratory of Longevity and Ageing-Related Disease of Chinese Ministry of Education,Guangxi Medical University,Nanning 530021)

机构地区：[1]广西医科大学国际教育学院,南宁530021 [2]广西医科大学转化医学研究中心,南宁530021 [3]广西医科大学基础医学院,南宁530021 [4]广西医科大学长寿与老年相关疾病教育部重点实验室,南宁530021

出　　处：《安徽医科大学学报》2022年第5期759-763,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81670750)。

摘　　要：目的探讨长期高血糖对链脲佐菌素诱导的1型糖尿病(T1DM)食蟹猴左心室心肌组织超微结构的影响。方法通过静脉注射链脲佐菌素诱导构建T1DM食蟹猴模型为模型组,正常食蟹猴为对照组,运用透射电镜观察模型组和对照组的左心室心肌组织超微结构并进行定量分析。结果与对照组相比,模型组左心室心肌细胞胞质中线粒体数量减少(P<0.01),单位线粒体面积内相对电子密度、线粒体体积密度、表面积密度均降低(P<0.0001,P<0.001,P<0.05),差异有统计学意义;而线粒体表面积体积比、线粒体平均面积差异无统计学意义;左心室心肌细胞胞质中脂滴增多,左心室心肌微血管内皮细胞吞饮小泡数量减少、基底膜增厚(P<0.0001)。结论长期高血糖引起T1DM食蟹猴左心室心肌细胞和心肌微血管内皮细胞结构受损。Objective To investigate the effects of long-term hyperglycemia induced ultrastructure of left ventricular myocardium in streptozotocin-induced type 1 diabetes mellitus(T1DM)cynomolgus monkeys.Methods The T1DM cynomolgus monkeys were induced by intravenous administration of streptozotocin as the model group.The normal cynomolgus monkeys were distributed to the control group.Ultrastructural morphometric features of left ventricular myocardium in the model group and the control group were obtained using transmission electron microscope and quantitatively analyzed.Results Ultrastructural observations indicated that the numbers of mitochondrion(P<0.01),the relative electron density of mitochondrion(P<0.0001),the volume density(P<0.001)and the surface area density(P<0.05)of mitochondrion were observed to decrease in the cytoplasm of left ventricular cardiomyocytes of the model group compared to the control group.The ration surface and the average area of mitochondrion showed no difference between two groups.Massive lipid droplets were identified in the cytoplasm of left ventricular cardiomyocytes of the model group.Thickening of basement membrane(P<0.0001)and decreasing of pinocytotic vesicles(P<0.0001)were observed in the capillary endothelial cells in left ventricular myocardium of the model group compared to the control group.Conclusion The results indicate that long-term hyperglycemia induces left ventricular cardiomyocytes and capillary endothelial cells ultrastructural damage in TIDM cynomolgus monkeys.

关 键 词：1型糖尿病 食蟹猴 心肌细胞 内皮细胞 超微结构 

分 类 号：R587.1[医药卫生—内分泌]