不同Sap活性白假丝酵母菌对阴道上皮局部免疫的影响  

Effects of Candida albicans with different Sap on host immunity of vaginal epithelial cells

作　　者：邵明琨[1] 侯梦瑶 罗丹丹 祁文瑾[2] Shao Mingkun;Hou Mengyao;Luo Dandan;Qi Wenjin(Dept of Obstetrics and Gynecology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101;Dept of Obstetrics and Gynecology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032;Dept of Obstetrics and Gynecology, Kunming Women and Children′s Health Center, Kunming 650000)

机构地区：[1]昆明医科大学第二附属医院妇产科,昆明650101 [2]昆明医科大学第一附属医院妇产科,昆明650032 [3]昆明市妇女儿童保健中心,昆明650000

出　　处：《安徽医科大学学报》2022年第5期791-795,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81660248)。

摘　　要：目的探讨不同Sap分泌型天冬氨酸蛋白酶(Sap)活性菌株对阴道局部免疫的影响。方法收集门诊外阴阴道假丝酵母菌病(VVC)及复发性外阴阴道假丝酵母菌病(RVVC)患者阴道分泌物进行菌株鉴定,取白假丝酵母菌行药敏性分析、25S rDNA基因分型、Sap及磷酯酶(Plb)活性检测;用不同Sap活性的白假丝酵母菌与阴道上皮细胞共培养,用ELISA方法检测细胞上清液中白细胞介素(IL)-4、IL-8、IL-17的水平。结果VVC白假丝酵母菌株的Sap活性强于RVVC菌株;白假丝酵母菌感染后,阴道上皮细胞分泌的IL-4、IL-8、IL-17更多,且IL-8和IL-17出现的时间早、持续时间长;Sap强的白假丝酵母菌能刺激阴道上皮分泌更多的细胞因子。结论除外Sap活性,VVC与RVVC菌株之间侵袭力无明显差异;白假丝酵母菌能在短时间内激活阴道宿主免疫反应,且Sap活性强的菌株诱发的宿主免疫也强;RVVC致病白假丝酵母菌Sap活性弱的原因可能与阴道上皮分泌的保护性因子有关。Objective To investigate the difference in invasiveness between vulvovaginal candidiasis(VVC)and recurrent vulvovaginal candidiasis(RVVC)strains,and to explore the effect of strains with different secreted aspartate protease(Sap)on vaginal local immunity.Methods Vaginal secretions of VVC and RVVC patients were collected for fungus identification.Candida albicans(C.albicans)were taken for drug sensitivity analysis,25S rDNA genotyping,and detection of secreted aspartate protease(Sap)and phospholipase(Plb)activity.C.albicans with different Sap activity were cultured with vaginal epithelial cells,and the levels of Interleukin(IL)-4,IL-8,IL-17 in cell supernatant were measured by ELISA.Results Sap activity of VVC C.albicans was stronger than that of RVVC.After infection with C.albicans,the secretion of IL-4,IL-8 and IL-17 by vaginal epithelial cells was higher,and IL-8 and IL-17 appeared earlier and lasted longer.C.albicans with strong Sap enzyme activity could stimulate vaginal epithelial cells to secrete more cytokines.Conclusion Except for the Sap activity,there was no significant difference in the invasiveness between VVC and RVVC strains.C.albicans could activate the vaginal host immune response in a short time.The lower Sap activity of C.albicans in RVVC patients may be related to the protective factors secreted by the vaginal epithelium.

关 键 词：外阴阴道假丝酵母菌病 白假丝酵母菌 分泌型天冬氨酸蛋白酶活性 IL-4 IL-8 IL-17 

分 类 号：R711.31[医药卫生—妇产科学]