隔药饼灸对动脉粥样硬化易损斑块兔腹主动脉PI3K、AKT、TNF-α表达的影响  

Effects of medicinal cake-separted moxibustion on PI3K, AKT and TNF-α of abdominal aorta in rabbits with vulnerable atherosclerotic plaque

作　　者：易丽贞 杨俊[1] 胡岗珠 刘欣 吴雪芬[1] 岳增辉[1] YI Li-zhen;YANG Jun;HU Gang-zhu;LIU Xin;WU Xue-fen;YUE Zeng-hui(School of Acupuncture,Moxibustion&Tuina Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410208

出　　处：《中华中医药杂志》2022年第4期1938-1941,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81874430);湖南省研究生创新项目(No.CX20200779,No.CX20200753,No.CX20200778)。

摘　　要：目的:观察隔药饼灸对动脉粥样硬化易损斑块兔腹主动脉组织中磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、肿瘤坏死因子-α(TNF-α)表达的影响。方法:随机将新西兰大耳白兔分为空白组、模型组、直接灸组、隔药饼灸组和药物组,每组12只,采用球囊损伤腹主动脉加高脂饲料喂养结合建立兔AS易损斑块模型。造模成功后,分别对直接灸组、隔药饼灸组、药物组进行艾柱灸、艾柱+药饼、口服阿托伐他汀钙片干预。采用免疫组化法和实时荧光定量PCR法检测兔腹主动脉PI3K、AKT、TNF-α的灰度值和基因表达。结果:与空白组比较,模型组PI3K、AKT、TNF-α灰度值表达显著下降(P<0.05,P<0.01),基因表达显著升高(P<0.05,P<0.01)。与模型组比较,药物组和隔药饼灸组兔PI3K、AKT的灰度值表达均显著上调(P<0.05,P<0.01),直接灸、隔药饼灸和药物组TNF-α的灰度值表达显著上调(P<0.05,P<0.01),直接灸组的PI3K、AKT、TNF-αmRNA的表达显著降低(P<0.05,P<0.01);隔药饼灸组AKT、TNF-αmRNA表达显著降低(P<0.01);药物组PI3K mRNA表达显著降低(P<0.05)。结论:隔药饼灸可通过抑制AKT阻断信号通路,降低炎性介质TNF-α表达,这可能为其稳定易损斑块的作用机制之一。Objective:To observe the effect of medicinal cake-separted moxibustion on the expression of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and tumor necrosis factor-α(TNF-α)in abdominal aorta of rabbits with atherosclerotic vulnerable plaque.Methods:New Zealand white rabbits were randomly divided into 5 groups,including blank group,model group,direct moxibustion group,medicinal cake-separted moxibustion group and western medicine group.Rabbits with AS vulnerable plaque model were fed with high-fat diet combined with balloon injury of abdominal aorta.After successful modeling,dispose of each group separately,the direct moxibustion group(moxibustion with moxa cone),medicinal cake-separted moxibustion group(moxa cone+medicine cake),western medicine group(Atorvastatin calcium).The gray values and gene expression of PI3K,AKT and TNF-αwere detected by immunohistochemistry and real-time quantitative PCR.Results:Compared with the blank group,the grey level expressions of PI3K,AKT and TNF-αin model group were significantly decreased(P<0.05,P<0.01),while the gene expression was significantly increased(P<0.05,P<0.01).Compared with the model group,the expression of PI3K,Akt grey level in western medicine group and medicinal cake-separted moxibustion group were significantly increased(P<0.05,P<0.01),the expression of TNF-αin western medicine group and medicinal cake-separted moxibustion group and direct moxibustion group were significantly increased(P<0.05,P<0.01),the expression of PI3K,AKT and TNF-αmRNA in direct moxibustion group was significantly decreased(P<0.05,P<0.01),the expression of AKT,TNF-αmRNA in medicinal cake-separted moxibustion group was significantly decreased(P<0.01),and the expression of PI3K mRNA in western medicine group was significantly decreased(P<0.05).Conclusion:The medicinal cake-separted moxibustion can reduce inflammatory medium TNF-αexpression by suppressing AKT blocking signal pathways,which may be one of the mechanisms of action of stabilizing vulnerable plaque.

关 键 词：动脉粥样硬化 易损斑块 隔药饼灸 磷脂酰肌醇3-激酶 蛋白激酶B 肿瘤坏死因子-α 

分 类 号：R245[医药卫生—针灸推拿学]