急性氨氮胁迫对长蛸鳃、肝脏和肾脏组织结构的影响  

Effects of Acute Ammonia Exposure on Histopathology of Gill,Liver and Kidney of Octopus minor

作　　者：陈智威 许然 郑小东[1,2] Chen Zhiwei;Xu Ran;Zheng Xiaodong(Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;The Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China)

机构地区：[1]中国海洋大学海洋生物多样性与进化研究所,山东青岛266003 [2]海水养殖教育部重点实验室(中国海洋大学),山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2022年第6期62-68,共7页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(31672257);中央高校基本科研业务费专项(201822022)资助。

摘　　要：为探究急性氨氮胁迫对长蛸(Octopus minor)组织结构的影响,本实验以长蛸成体为研究对象,设置对照组(0 mg/L)和低(87.0 mg/L)、中(143.5 mg/L)、高(204.0 mg/L)氨氮浓度组,对长蛸胁迫48 h后,观察其鳃、肝脏和肾脏组织结构。研究发现,随着氨氮胁迫浓度升高,肾细胞核直径无明显差异,但细胞不断缩小,肾小管之间空隙变大,有大量组织液渗出;肝细胞核直径逐渐增大,细胞排列逐渐松散,中央静脉管壁的完整度逐渐降低,肝组织的部分结构损坏;鳃细胞核直径逐渐增大,排列松散,部分细胞核开始溶解,空泡化不断加剧。研究结果显示,组织结构损伤程度与氨氮胁迫浓度呈正相关,氨氮胁迫浓度越高,长蛸的组织结构损伤程度越明显,其中肾脏损伤程度较大,肝脏和鳃次之。In order to explore the effect of ammonia nitrogen stress on the tissue structure of Octopus minor,we used four ammonia nitrogen concentrations,control(0 mg/L),low(87.0 mg/L),medium(143.5 mg/L),and high(204.0 mg/L),to stress O.minor for 48h.Gills,liver and kidneys were used for histological microsection analysis.Microscopic examination revealed that with the increase of ammonia nitrogen concentration,there was no significant difference in the diameter of cell nucleus,but the cells of kidneys continued to shrink.The gaps between the kidney tubules became larger than the original,and a large amount of tissue fluid exuded.The diameter of the liver cell nucleus gradually increased,and these cells arranged loosely.The structure of the central vein in the liver was damaged.The gill cell nuclei gradually increased,and some of them were dissolved and vacuolated.The results of this study provided a theoretical basis for in-depth research on the metabolic mechanism of ammonia detoxification of the animals with O.minor as a representative.The results of this study showed that the degree of tissue damage was positively correlated with the heaviness of ammonia stress.Increasing concentration of ammonia caused serious damages of tissues,among them kidneys were damaged most seriously,which were followed by liver and gills.

关 键 词：长蛸 氨氮胁迫 组织结构 鳃 肝脏 肾脏 

分 类 号：S917[农业科学—水产科学]