基于灰色关联度分析法的多花黄精不同炮制品水煎液滋阴作用与其成分相关性研究  

Correlation Between the Yin-nourishing Effect of Aqueous Decoction of Polygonatum Cyrtonema Hua Processed Products and Its Components Based on Grey Relational Analysis

作　　者：余欢迎 高海燕 金传山[1,2] 张亚中 许凤清[1,2] 张伟 王桐生[1,2] 柳春风 李雷[5] YU Huanying;GAO Haiyan;JIN Chuanshan;ZHANG Yazhong;XU Fengqing;ZHANG Wei;WANG Tongsheng;LIU Chunfeng;LI Lei(Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology,Hefei 230012,China;Anhui Province Institute for Food and Drug Control,Hefei 230051,China;Jinzhai Runyuan Biological Technology Co.,Ltd.,Lu′an 237300,China;Jinzhai Senfeng Agricultural Technology Development Co.,Ltd.,Lu′an 237000,China)

机构地区：[1]安徽中医药大学,合肥230012 [2]中药饮片制造新技术安徽省重点实验室,合肥230012 [3]安徽省食品药品检验研究院,合肥230051 [4]金寨润元生物科技有限公司,六安237300 [5]金寨森沣农业科技开发有限公司,六安237000

出　　处：《世界中医药》2022年第9期1220-1226,共7页World Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1707200);安徽省高等学校自然科学研究项目(KJ2020A0421);六安市产学研合作重大专项(六科[2019]19)。

摘　　要：目的:研究多花黄精不同炮制品水煎液指纹图谱及小分子糖类对其滋阴作用贡献大小顺序,建立“黄精水煎液化学成分与药效关联”的黄精质量控制模式。方法:采用灰色关联度分析方法,研究多花黄精不同炮制品水煎液高效液相色谱法(HPLC)指纹图谱及小分子糖类共有峰峰面积与环核苷酸系统比值、皮质酮以及肌酐的相关性,建立多花黄精不同炮制品水煎液滋阴作用的“谱效”关系。结果:多花黄精不同炮制品对氢化可的松致大鼠肾阴虚具有显著的改善作用,与黄精传统“滋阴”功效一致。多花黄精不同炮制品水煎液的HPLC指纹图谱中,16、1、36(果糖)、22(黄精碱A)、14、31、37(葡萄糖)号色谱峰所代表的化学成分与滋阴作用相关性较强,贡献较大,特别是36、22号色谱峰,随着蒸晒次数的增加,谱图中峰面积增加、关联度增强,可考虑作为黄精含量测定相关指标,进而完善黄精饮片的质量标准。结论:多花黄精不同炮制品水煎液与其滋阴作用之间有一定的对应关系,其水煎液HPLC指纹图谱在一定程度上可以反映黄精饮片的质量特征,炮制品中黄精碱A、果糖和葡萄糖与滋阴作用有较强的相关性。Objective:To study the fingerprints of aqueous decoction of different Polygonatum cyrtonema processed products and the contribution of small molecular carbohydrates to the yin-nourishing effect,and to establish a quality control model based on the correlation between the chemical components of aqueous decoction of P.cyrtonema processed products and its efficacy.Methods:Grey relational analysis was used to study the HPLC fingerprints of aqueous decoction of different P.cyrtonema processed products and the correlation between common peak areas of small molecule carbohydrates and cyclic nucleotide ratio,corticosterone,creatinine.The spectrum-effect relationship of the yin-nourishing effect of aqueous decoction of different processed products was established.Results:Different P.cyrtonema processed products had significant improvement effects on hydrocortisone-induced kidney yin deficiency in rats,which was consistent with the traditional yin-nourishing effect of P.cyrtonema.In the HPLC fingerprints of aqueous decoction of different P.cyrtonema processed products,the chemical components represented by peaks 16,1,36(fructose),22(polygonatine A),and 14,31,37(glucose)were closely related to nourishing yin,with large contribution,especially peaks 36 and 22.With the increase in the number of steaming and drying,the peak area in the spectrum and the correlation degree increased,which could be considered as indicators to determine the content of P.cyrtonema and further improve the quality standard of P.cyrtonema decoction pieces.Conclusion:There was a corresponding relationship between the aqueous decoction of different P.cyrtonema processed products and its yin-nourishing effect.The HPLC fingerprint of the aqueous decoction could reflect the quality characteristics of P.cyrtonema decoction pieces to a certain extent,and polygonatine A,fructose,glucose were closely correlated with the yin-nourishing effect.

关 键 词：黄精 指纹图谱 滋阴 谱效关系 

分 类 号：R285.5[医药卫生—中药学]