烟草黑胫病生防细菌的鉴定和防效测定  

Identification and control efficiency of biocontrol bacteria against tobacco black shank

作　　者：张蒙蒙 梁俊阳 王清福 都亚飞 张洁[4] 刘前进 彭建斐 付博[1] ZHANG Mengmeng;LIANG Junyang;WANG Qingfu;DU Yafei;ZHANG Jie;LIU Qianjin;PENG Jianfei;FU Bo(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450002,China;Technology Center,China Tobacco Henan Industrial Co.,Litd.Zhengzhou 450016 China;Huaihua Branch of Hunan Provincial Tobacco Company,Huaihua 418000,China;Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]河南中烟工业有限责任公司技术中心,河南郑州450016 [3]湖南省烟草公司怀化市公司,湖南怀化市418000 [4]河南省农业科学院植物保护研究所,河南郑州450002

出　　处：《河南农业大学学报》2022年第2期219-227,共9页Journal of Henan Agricultural University

基　　金：湖南省烟草公司怀化市公司科技项目(JSCX-2020-007);国家自然科学基金项目(31700270)。

摘　　要：【目的】挖掘烟草黑胫病的生防资源,通过试验对前期分离到的2株生防菌进行种类鉴定和防治效果测定。【方法】根据其形态学、生理生化特征和16 S rDNA序列分析,YCYM-04菌株被鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia),YCYM-09菌株被鉴定为贝莱斯芽孢杆菌(Bacillus velezensis)。【结果】平板对峙结果显示,YCYM-04菌株和YCYM-09菌株对烟草疫霉的抑菌带分别达13.7和8.9 mm。此外,YCYM-04菌株和YCYM-09菌株发酵滤液5倍稀释液菌落抑菌率分别达71.8%和50.6%;YCYM-04菌株发酵滤液造成烟草疫霉菌丝节间缩短、断裂、生长缓慢等现象,YCYM-09菌株发酵滤液造成烟草疫霉菌丝体膨大、增粗、畸形、原生质体凝结。盆栽试验表明,YCYM-04菌株和YCYM-09菌株培养液灌根处理对烟草黑胫病的防治效果分别达50.5%和61.5%。【结论】YCYM-04和YCYM-09菌株在烟草黑胫病的生物防治上具有潜在的应用价值。【Objective】In order to explore new biocontrol resources against tobacco black shank,two biocontrol bacteria were identified and their control efficiency was determined.【Method】Based on morphology,physiological and biochemical characteristics and 16S rDNA sequence analyses,strain YCYM-04 and YCYM-09 were identified as Stenotrophomonas maltophilia and Bacillus velezensis,respectively.【Result】The results of confrontation culture showed that the inhibition zone widths of YCYM-04 and YCYM-09 against Phytophthora nicotianae were 13.7 mm and 8.9 mm,respectively.In addition,the inhibition rate of colony growth treated by the 5 times diluted of YCYM-04 and YCYM-09 fermentation filtrate reached 71.8%and 50.6%,respectively.The mycelium of the pathogen of Phytophthora nicotianae treated by strain YCYM-04 fermentation filtrate showed shortened internodes,fractured,and slowly growth,while it showed inflated,incrassate,malformed and protoplast condensation when treated by strain YCYM-09 fermentation filtrate.Pot experiment showed that the control effect of strain YCYM-04 and YCYM-09 on tobacco black shank could reach 50.5%and 61.5%,respectively.【Conclusion】Strain YCYM-04 and YCYM-09 possess potential applications on the biocontrol of tobacco black shank.

关 键 词：烟草 黑胫病 生物防治 细菌 鉴定 防治效果 

分 类 号：S572[农业科学—烟草工业] S435.72[农业科学—作物学]