二代测序技术诊断糖尿病酮症酸中毒患者肺部金黄色葡萄球菌感染一例报道并文献复习  

Diagnosis of Pulmonary Staphylococcus Aureus Infection in Patients with Diabetic Ketoacidosis by Next-generation Sequencing Technology:a Case Report and Literature Review

作　　者：王茜[1] 王岷[2] 吴文颖 梁冉冉 WANG Qian;WANG Min;WU Wenying;LIANG Ranran(Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Department of Respiratory and Critical Care,the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,China)

机构地区：[1]山东中医药大学,山东省济南市250014 [2]山东中医药大学第二附属医院呼吸与危重症医学科,山东省济南市250001

出　　处：《实用心脑肺血管病杂志》2022年第6期136-140,共5页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

摘　　要：二代测序(NGS)是一种新的分子生物学技术。本文报道1例糖尿病酮症酸中毒伴肺部影像学明显改变患者,经痰培养、痰病理检查等不能明确病原体,经验性用药效果不佳,后通过支气管肺泡灌洗液(BALF)行NGS确诊为肺部金黄色葡萄球菌感染,调整治疗方案后患者病情好转出院,这为临床上常规检查不能明确病原体、经验性抗感染治疗效果不佳的肺部感染患者的诊疗提供一定借鉴。Next-generation sequencing(NGS)is a new molecular biology technology.This paper reports a case of diabetic ketoacidosis with obvious changes in lung imaging.The pathogen could not be identified by sputum culture and sputum pathology,and the empirical medication effect was poor.Ultimately,the diagnosis of pulmonary aureus infection was confirmed by NGS of bronchoalveolar lavage fluid,and the treatment regimen was adjusted,resulting in a favorable outcome.This provides some reference for the diagnosis and treatment of patients with pulmonary infection who can not identify the pathogen by routine clinical examination and has poor effect of empirical anti infection treatment.

关 键 词：肺炎 肺部感染 糖尿病酮症酸中毒 金黄色葡萄球菌 二代测序 病例报告 

分 类 号：R563.1[医药卫生—呼吸系统]