拟南芥F-box蛋白FKF1与转录因子FUL互作调控开花研究  

Study on the Interaction of F-box Protein FKF1 and Transcription Factor FUL in Regulating Flowering in Arabidopsis

作　　者：周娟 阎晋东 李新梅 刘雪晴 赵强 赵小英 ZHOU Juan;YAN Jin-dong;LI Xin-mei;LIU Xue-qing;ZHAO Qiang;ZHAO Xiao-ying(Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan Hybrid Rapeseed Engineering Technology Research Center,College of Biology,Hunan University,Changsha 410082)

机构地区：[1]湖南大学生物学院植物功能基因组学与发育调控湖南省重点实验室湖南省杂交油菜工程技术研究中心,长沙410082

出　　处：《生物技术通报》2022年第3期1-8,共8页Biotechnology Bulletin

基　　金：湖南省自然科学基金项目(2020JJ4183);深圳市科技计划项目(JCYJ20170818112212721);长沙市科技计划项目(kq1901028)。

摘　　要：F-box蛋白FLAVIN-BINDING KELCH REPEAT F-BOX 1(FKF1)参与调控拟南芥光周期开花,但其分子机制尚不完全清楚。本研究通过体内和体外实验,证明FKF1与转录因子FRUITFULL(FUL)相互作用。qRT-PCR和Western blot结果显示,FKF1正调节FUL的转录水平,但不影响FUL蛋白的稳定性。遗传分析结果显示,35S-FKF1-Myc/ful-8双突变体的开花表型以及开花基因FLOWERING LOCUS T(FT)的转录水平与ful-8突变体相一致。研究结果表明FKF1可通过与FUL互作,在FUL的上游促进FT表达,进而促进开花。F-box protein FLAVIN-BINDING KELCH REPEAT F-BOX 1(FKF1)is involved in regulating photoperiod flowering in Arabidopsis;however,the underling molecular mechanism is not fully understood yet.Here,we confirmed that FKF1 interacted with transcription factor FRUITFULL(FUL)in vivo and in vitro.qRT-PCR and Western blot results indicated that FKF1 positively regulated the FUL expression in transcriptional level,but had no influence on the stability of FUL protein.Genetic analyses indicated that the flowering phenotype of 35S-FKF1-Myc/ful-8 double mutants and the transcription level of flowering integrator gene FLOWERING LOCUS T(FT)in them were consistent to that in the ful-8 mutant.Collectively,these findings indicate that FKF1 interacts with FUL and functions the upstream of FUL to promote FT expression,thereby promoting flowering.

关 键 词：开花 FKF1 FUL 拟南芥 

分 类 号：Q943.2[生物学—植物学]