红汁乳菇多糖的结构特征及对肠道菌群的影响  

Structural characteristics of polysaccharides from Lactarius hatsudake and its effect on intestinal flora

作　　者：张馨方 程孟雅 杨桥 常松林 任佳丽 ZHANG Xin-fang;CHENG Meng-ya;YANG Qiao;CHANG Song-lin;REN Jia-li(Hunan Key Laboratory of Forestry Edible Sources Safety and Processing,Changsha,Hunan 410004,China;College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004,China)

机构地区：[1]林产可食资源安全与加工利用湖南省重点实验室,湖南长沙410004 [2]中南林业科技大学食品科学与工程学院,湖南长沙410004

出　　处：《食品与机械》2022年第5期143-148,共6页Food and Machinery

基　　金：湖南省创新平台与人才计划项目(编号:2019TP1029);湖南省重点研发计划项目(编号:2022SK2100,2020SK2100)。

摘　　要：目的:探究红汁乳菇多糖对肠道菌群的调节作用。方法:以红汁乳菇(Lactarius hatsudake)为原料,通过热水提取获得红汁乳菇多糖LHP,进一步纯化得到LHP-1和LHP-2,对其基础结构进行表征,并通过抗生素诱导小鼠模型,研究LHP对小鼠体重和肠道微生物群组成的影响。结果:LHP-1主要由甘露糖、葡萄糖、半乳糖组成,其摩尔质量比为3.44∶61.10∶1.22;LHP-2主要由甘露糖和葡萄糖组成,其摩尔质量比为3.84∶153.00,具有“神经元”结构和链状结构区域。LHP的干预能通过增加拟杆菌属(Bacteroides)、阿克曼菌属(Akkermansia)等有益菌和减少肠球菌属(Enterococcus)、短杆菌属(Brevundimonas)等有害菌来恢复肠道菌群的结构和多样性,特别是有益菌嗜黏蛋白—阿克曼菌(A.muciniphila)的增加表明了LHP在人体肠道菌群调节中的巨大潜力。结论:红汁乳菇多糖能够作为潜在益生元改善肠道代谢。Objective:This study aimed to explore the regulatory effect of Lactarius hatsudake polysaccharide on intestinal flora.Methods:LHP was extracted from L.hatsudake by hot water.LHP-1 and LHP-2 were further purified and characterized.The effects of LHPs on body weight and intestinal microbiome composition were studied in antibiotic-induced mice.Results:LHP-1 was mainly composed of mannose,glucose and galactose.The molar mass ratio of LHP-1 was 3.44∶61.10∶1.22.LHP-2 is mainly composed of mannose and glucose,with a molar mass ratio of 3.84∶153.00.It has a"neuronal"structure and a chain structure region.LHP intervention can restore the structure and diversity of intestinal flora by increasing beneficial bacteria such as Bacteroides and Akkermansia and reducing harmful bacteria such Enterococcus and Brevundimonas.In particular,the increase of the beneficial bacteria A.muciniphila indicates the great potential of LHP in the regulation of human intestinal flora.Conclusion:Polysaccharide from Lactarius hatsudake can improve intestinal metabolism as potential prebiotics.

关 键 词：红汁乳菇 多糖 结构 肠道菌群 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]