三键的拉曼强度编码聚合物的制备及细胞成像的研究  

Preparation of Raman Active Polymer with Encoding of Triple Bonded Raman Intensity and Cell Imaging

作　　者：朱伟 沈爱国 胡继明[2] ZHU Wei;SHEN Aiguo;Hu Jiming(School of Printing and Packaging, Wuhan University, Wuhan, 430079, China;College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China)

机构地区：[1]武汉大学印刷与包装系,武汉430079 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《光散射学报》2022年第1期40-45,共6页The Journal of Light Scattering

基　　金：国家自然科学基金项目(22074109,21874102);中国博士后科学基金(2021M702506)。

摘　　要：用于生物多元标记的三键拉曼散射标签引起了研究者广泛关注,然而三键标签难以突破“颜色极限”,且基于三键拉曼位移不同的信号分子合成难度大、分子标签的拉曼信背比较差。本文主要通过对含有强三键拉曼散射的小分子单体进行合理裁剪,在纳米级水平上进行聚合,通过人造“指纹”输出的更小但更亮的光学纳米标签,提高了生物成像的对比度。尤其是通过调节三种不同化学性质的三键单体在共聚合过程中的相对剂量,设计了一类富三键聚合物纳米粒子,通过三键的拉曼强度编码策略制备出了十五种可区分的三键比率型输出标签。纳米分子结构聚集物的精确构建,将成为小尺寸、高灵敏度和高通量生物成像标签多色成像的通用方法。Triple-bond based-Raman scattering tags have attracted extensive researchers’attention for biological multivariate labeling.However,there are still difficult to break through the“color limit”,the synthesis of signal molecules based on Raman shifts of triple bond is difficult,and the signal noise ratio of small Raman molecules is poor.In this paper,small molecular monomers containing strong triple-bond Raman scatters were rationally tailored and polymerized at the nanoscale level,which could enhance imaging contrast with smaller but brighter optical nanotags with artificial‘fingerprint’output.In particular,a class of triple-bond rich polymer nanoparticles(NPs)has been engineered by regulating the relative dosages of three chemically different triple-bond monomers in co-polymerization,and the suggested bonding can achieve 15 spectrally distinguishable triple-bond combinations.The accurate construction of structured nano molecular aggregates will become a universal method for small-size,high-sensitivity and high-throughput biological imaging tags for multi-color imaging.

关 键 词：三键 聚合物 拉曼强度 成像 

分 类 号：O65[理学—分析化学]