运动疲劳引发的大鼠纹状体神经元树突棘可塑性变化及其细胞骨架机制  

Plasticity changes of dendritic spines and cytoskeletal mechanisms of striatum neurons induced by exercise fatigue in rats

作　　者：龚云[1,2] 刘晓莉[1] 乔德才[1] GONG Yun;LIU Xiao-li;QIAO De-cai(College of Physical Education and Sports,Beijing Normal University,Beijing 100875, China;College of Physical Education,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]西北师范大学体育学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2022年第3期91-99,共9页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省体育科研和决策咨询研究项目(GST2020YB02)。

摘　　要：为了探讨运动疲劳引起的大鼠纹状体神经元树突棘的可塑性变化及其细胞骨架机制,将20只雄性Wistar大鼠随机分为2组:对照组(CG,n=10)和实验组(PG,n=10).PG组大鼠以递增负荷跑台运动建立运动疲劳模型,实验末检测体重和血糖,制备纹状体高尔基染色切片,数码光学显微镜观察树突棘的形态并拍照,Image J软件测量其密度、数量及类型.通过实时荧光定量聚合酶链式反应(qRT-PCR)技术,检测纹状体神经元纤维状肌动蛋白(F-actin)、微管相关蛋白(MAP_(2),Tau)基因表达情况.结果表明,与CG组比较,PG组大鼠血糖显著升高(P<0.05),树突棘蘑菇型占比极显著增高(P<0.01),短粗型占比极显著下降(P<0.01),树突棘密度和数量均显著增高(P<0.05);同时,PG组大鼠F-actin,MAP_(2)和Tau基因相对表达量分别是CG组的1.33,1.06和1.71倍.据此推断,运动疲劳引发的纹状体神经元树突棘可塑性变化归因于该部神经元细胞骨架蛋白相关基因表达的上调.In order to discuss the plasticity changes of dendritic spines and cytoskeleton mechanism on striatal neurons induced by exercise fatigue in rats,twenty male Wistar rats were randomly divided into two groups,that is control group(CG,n=10)and experimental group(PG,n=10).The exercise fatigue model was established through treadmill running with increasing load in PG group rats.At the end of the experiment,weight and blood glucose was detected,Golgi staining tissue sections were made,the morphological characteristics of dendritic spine was observed,and photographed by digital optical microscope,then to measure its density,number and types by ImageJ software.The expression of genes about F-actin,MAP_(2) and Tau in striatal neurons was detected by qRT-PCR.Results showed that,compared with CG group,the blood glucose of PG group was significantly increased(P<0.05).The proportion of mushroom type of dendritic spines was very significantly increased(P<0.01),while the proportion of stubby type was very significantly decreased(P<0.01).The density and number of dendritic spines were increased significantly(P<0.05)in PG group.The relative expression levels of F-actin,MAP_(2) and Tau genes in PG group respectively were 1.33,1.06 and 1.71 times than CG group.On the basis of reasoning,the plasticity changes of dendritic spine induced by exercise fatigue in striatum neurons were attributed to up-regulation of cytoskeletal protein related gene expression.

关 键 词：运动疲劳 纹状体 树突棘 突触可塑性 细胞骨架 大鼠 

分 类 号：Q189[生物学—神经生物学]