褐飞虱对氟啶虫胺腈的抗性监测与生化抗性机制  

Resistance monitoring and biochemical resistance mechanism of Nilaparvata lugens to sulfoxaflor

作　　者：裴新国 张帅 张彦超 郑晨 高聪芬[1] 吴顺凡 PEI Xinguo;ZHANG Shuai;ZHANG Yanchao;ZHENG Chen;GAO Congfen;WU Shunfan(College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China)

机构地区：[1]南京农业大学植物保护学院,南京210095

出　　处：《植物保护》2022年第3期39-46,共8页Plant Protection

基　　金：国家重点研发计划(2018YFD0200300);江苏省农业科技自主创新资金(CX[19]3003)。

摘　　要：为明确我国褐飞虱田间种群对氟啶虫胺腈的抗性现状及生化抗性机制,2017年-2019年采用稻茎浸渍法测定了采集自7省共13个褐飞虱田间种群对氟啶虫胺腈的抗性,并研究了氟啶虫胺腈抗性种群与其他杀虫剂的交互抗性以及增效剂对氟啶虫胺腈的增效效果。结果表明:近3年来褐飞虱对氟啶虫胺腈产生了中等水平抗性(RR=10.3~30.9)。氟啶虫胺腈抗性品系对呋虫胺、噻虫嗪和烯啶虫胺分别产生了9.1倍、7.9倍和4.1倍的低水平交互抗性,与噻嗪酮、毒死蜱、吡蚜酮、三氟苯嘧啶和吡虫啉不存在交互抗性。增效剂PBO对氟啶虫胺腈抗性品系和浙江龙游19(Longyou-19)田间种群分别具有4.2倍和3.8倍的明显增效作用。综上,褐飞虱田间种群已对氟啶虫胺腈产生中等水平抗性。多功能氧化酶参与了褐飞虱对氟啶虫胺腈的代谢抗性。To understand the resistance status and mechanism of field populations of Nilaparvata lugens to sulfoxaflor,we determined the resistance to sulfoxaflor of 13 field populations of N.lugens collected from seven provinces in 2017-2019 by using the rice-stem dipping method.Cross-resistance and synergism tests were also investigated in this study.The results showed that all of the field populations had developed moderate resistance to sulfoxaflor(resistance ratio=10.3-30.9).Compared with the sensitive strain(SUL-S),the selected SUL-R strains showed low-level cross-resistance to dinotefuran,thiamethoxam and nitenpyram(9.1-fold,7.9-fold and 4.1-fold,respectively).No cross-resistance to buprofezin,chlorpyrifos,pymetrozine,triflumezopyrim and imidacloprid was detected.Bioassays revealed that the P450 inhibitor,piperonyl butoxide(PBO)had 4.2-fold and 3.8-fold synergistic effects on sulfoxaflor to sulfoxaflor-resistant strains and field strains(Longyou-19).Our results indicated that all of the populations developed moderate resistance to sulfoxaflor.Enhanced metabolism mediated by cytochrome P450 monooxygenases was involved in the resistance of N.lugens to sulfoxaflor.

关 键 词：褐飞虱 氟啶虫胺腈 抗药性监测 交互抗性 生化抗性机制 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]