朱红毛斑蛾和花胸姬蜂对虫害诱导榕树挥发物的触角电位及行为反应  

Electroantennographic and behavioral responses of Phauda flammans adults and its parasitoid Gotra octocinctus to ficus volatiles induced by P.flammans larvae

作　　者：管维康 王小云 陆温[1] 张志林 郑霞林[1] GUAN Weikang;WANG Xiaoyun;LU Wen;ZHANG Zhilin;ZHENG Xialin(Guangxi Key Laboratory of Agri-Environmental and Agri-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China;Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Xiaogan432000, China;College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China)

机构地区：[1]广西农业环境与农产品安全重点实验室,广西大学农学院,南宁530004 [2]特色果蔬质量安全控制湖北省重点实验室,孝感432000 [3]湖北工程学院生命科学技术学院,孝感432000

出　　处：《植物保护》2022年第3期81-89,共9页Plant Protection

基　　金：国家自然科学基金(31660206)。

摘　　要：朱红毛斑蛾Phauda flammans是一种寡食性害虫,仅取食小叶榕Ficus concinna和垂叶榕F.benjamina。花胸姬蜂Gotra octocinctus是其幼虫的优势寄生性天敌。研究朱红毛斑蛾及花胸姬蜂对朱红毛斑蛾为害小叶榕和垂叶榕后诱导产生的挥发物的触角电位及行为反应可为实施“推-拉”策略防控朱红毛斑蛾提供基础资料。基于气相色谱-质谱联用(gas chromatography-mass spectrum,GC-MS)和气相色谱-触角电位联用(gas chromatography-electroantennagraphic detection,GC-EAD),从受朱红毛斑蛾幼虫为害的小叶榕和垂叶榕虫害叶挥发物中筛选出对朱红毛斑蛾和花胸姬蜂雌雄成虫有电生理活性的物质,基于Y形嗅觉仪,测定朱红毛斑蛾和花胸姬蜂雌雄成虫对虫害叶挥发物成分的行为反应。结果表明,朱红毛斑蛾为害小叶榕和垂叶榕后,虫害叶中共分离出57种挥发物成分,其中,共有27种能引起朱红毛斑蛾雌雄虫的触角电位反应,在小叶榕和垂叶榕虫害叶片挥发物中同时存在的有11种。其中,两种榕树虫害叶特有的5种挥发物(β-月桂烯、β-罗勒烯、(-)-α-蒎烯、β-石竹烯和α-金合欢烯)均能引起花胸姬蜂雌雄成虫的触角电位反应。与健康叶挥发物混合物相比,受朱红毛斑蛾幼虫为害后释放的挥发物成分混合物对朱红毛斑蛾和花胸姬蜂雌雄成虫均具有更强的吸引作用。结果提示在生产上可使用朱红毛斑蛾为害后诱导的榕树挥发物成分混合物实施“推-拉”防控策略。Phauda flammans(Walker)(Lepidoptera:Phaudidae)is an oligophagous pest,which only feeds on Ficus concinna Miq.and F.benjamin L.Gotra octocinctus Ashmead is the dominant parasitoid of P.flammans larvae.Understanding the function of plant volatiles induced by P.flammans larvae from the two Ficus species can provide the basis for implementation of the“push-pull”control strategy against this moth.Gas chromatography-mass spectrum(GC-MS)and gas chromatography-electroantennographic detection(GC-EAD)were used to screen electrophysiological active substances from infested leaves of F.concinna and F.benjamina.Based on the Y olfactometer,the behavioral responses of male and female adults of P.flammans and G.octocinctus to plant volatiles from infested leaves were measured.A total of 57 compounds were isolated from F.concinna and F.benjamina leaves after damaged by P.flammans larvae.Male and female P.flammans exhibited electroantennographic responses to 27 compounds,of which 11 compounds were shared by F.concinna and F.benjamina.The five volatiles(β-myrcene,β-ocimene,(-)-α-copaene,β-caryophyllene andα-farnesene)specific to the leaves of two Ficus species damaged by P.flammans larvae could elicit electroantennographic response of male and female G.octocinctus.Compared with the volatile mixture from healthy Ficus leaves,the mixture from damaged leaves showed stronger attraction to both male and female adults of P.flammans and G.octocinctus.The results suggested that the volatile mixtures from damaged leaves of the two Ficus species have the potential for implementing the“push-pull”control strategy against P.flammans.

关 键 词：虫害诱导挥发物 朱红毛斑蛾 花胸姬蜂 触角电位 行为反应 

分 类 号：S763.3[农业科学—森林保护学]