复合性状转基因棉对斜纹夜蛾幼虫取食行为及营养代谢的影响  

Effects of stacked genetically modified cotton on the feeding behavior and nutrient metabolism of Spodoptera litura larvae

作　　者：丛胜波[1] 许冬[1] 杨妮娜[1] 王玲[1] 王金涛[1] 刘卫国 万鹏[1] CONG Shengbo;XU Dong;YANG Nina;WANG Ling;WANG Jintao;LIU Weiguo;WAN Peng(Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Disease Insect Pests and Weed, Wuhan 430064, China;Hubei General Station of Plant Protection, Wuhan 430070, China)

机构地区：[1]农业农村部华中作物有害生物综合治理重点实验室,农作物重大病虫草害防控湖北省重点实验室,湖北省农业科学院植保土肥研究所,武汉430064 [2]湖北省植保总站,武汉430070

出　　处：《植物保护》2022年第3期104-110,共7页Plant Protection

基　　金：转基因生物新品种培育重大专项(2016ZX08011002-002);湖北省农业科技创新中心项目(2016-620-003-001-016)。

摘　　要：本文采用培养皿叶盘法和烘干法研究了常规棉‘Daiza 24C’、单价抗虫棉‘GK19’和复合性状转基因抗虫棉‘Daiza 24’对斜纹夜蛾幼虫选择、取食及营养代谢的影响。结果表明,在非选择性条件下,虽然有较多的斜纹夜蛾1龄和3龄幼虫在‘Daiza 24’叶片上停留,但对其的取食量均显著低于‘Daiza 24C’和‘GK19’,表现出明显的拒食现象。在可选择条件下,取食‘Daiza 24’的1龄和3龄幼虫比例均随时间延长呈逐渐下降趋势,对‘Daiza 24’叶片表现出显著忌避行为,趋向于取食‘Daiza 24C’和‘GK19’。‘Daiza 24’对斜纹夜蛾3龄幼虫的相对生长率、相对取食量、食物利用率、食物转化率、近似消化率均存在显著影响,而对5龄幼虫,取食‘Daiza 24’的幼虫除相对生长率和相对取食量显著低于取食‘Daiza 24C’‘GK19’的幼虫外,其他营养指标在取食不同品种间差异均不显著。以上研究结果表明,斜纹夜蛾1龄和3龄幼虫对‘Daiza 24’表现出显著的拒食和忌避行为,‘Daiza 24’对斜纹夜蛾低龄幼虫的营养代谢存在显著影响,但对高龄幼虫营养代谢的影响较弱。The effects of conventional cotton‘Daiza 24C’,transgenic cotton‘GK19’and stacked genetically modified cotton‘Daiza 24’on feeding,host-selection behavior and nutrient metabolism of Spodoptera litura larvae were studied by using petri dish method and oven drying method.The results of non-choice experiments showed that although there were more 1st-and 3rd-instar larvae of S.litura stayed on the leaves of‘Daiza 24’,but their food intake was significantly lower than that of the larvae feeding on‘Daiza 24C’and‘GK19’,showing obvious antifeeding phenomenon.The choice experiments results demonstrated that the percentage of the 1st-and 3rd-instar larvae feeding on‘Daiza 24’decreased gradually with the time,and the larvae tended to choose‘Daiza 24C’and‘GK19’.Feeding on‘Daiza 24’had significant effects on the relative growth rate,relative food intake,food utilization efficiency,food conversion rate and approximate digestibility of the 3rd-instar larvae.However,there were no significant differences in other nutritional indexes among larvae feeding on three hosts for 5th-instar larvae,except for the relative growth rate and relative food intake.In summary the 1st-and 3rd-instar larvae of S.litura showed significant antifeeding and avoidance behaviors to‘Daiza24’.‘Daiza24’had significant effects on the nutrient metabolism of young larvae of S.litura,but weak effect on older larvae.

关 键 词：复合性状转基因棉花 斜纹夜蛾 取食行为 营养代谢 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]