草地贪夜蛾雄蛾交配状态的判断  

Determination of the mating status of male adult Spodoptera frugiperda

作　　者：冯行利 曹婷婷 杨凤丽 吴慧明[1] 饶琼[1] 郑永利 FENG Xingli;CAO Tingting;YANG Fengli;WU Huiming;RAO Qiong;ZHENG Yongli(College of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;Zhejiang Plant Protection Quarantine and Pesticide Management Station, Hangzhou 310020, China;Deqing County Agricultural Technology Extension Center, Huzhou City, Zhejiang Province, Huzhou 312300, China;Zhejiang Agricultural Products Quality and Safety Center, Hangzhou 310003, China)

机构地区：[1]浙江农林大学农业与食品科学学院,杭州311300 [2]浙江省植保检疫与农药管理总站,杭州310020 [3]浙江省湖州市德清县农业技术推广中心,湖州312300 [4]浙江省农产品质量安全中心,杭州310003

出　　处：《植物保护》2022年第3期159-164,共6页Plant Protection

基　　金：浙江省植物保护检疫总站项目(H20190332);杭州市农业与社会发展科研一般项目(20201203B160)。

摘　　要：草地贪夜蛾Spodoptera frugiperda是近年入侵我国的重大迁飞性害虫。本研究通过解剖观察不同日龄和交配状态的草地贪夜蛾雄蛾内生殖系统,确定其内含物分级标准,并以此为依据分析性诱剂田间诱捕雄蛾的交配状态。结果表明草地贪夜蛾精巢体积随日龄增加而减小,发育后期已交配的精巢皱缩扁平,日龄对未交配雄蛾单射精管非角质化区(PS)末端内含物无显著影响。根据PS末端内含物的颜色和紧密程度将雄蛾交配状态分为5级(Ⅰ~Ⅴ级)。未交配雄蛾PS末端内含物为褐色且均匀(Ⅴ级),交配后0 h呈透明(Ⅰ级),之后内含物会逐渐回填(Ⅱ~Ⅳ)。田间诱捕雄蛾共921头,其中647头已交配(Ⅰ~Ⅳ级)。不同性诱剂诱捕雄蛾的交配状态无显著差异。本研究结果为草地贪夜蛾的预测预报和性诱剂防控实际控害效果评价提供参考。The fall armyworm,Spodoptera frugiperda is an important invasive migratory pest in China.The internal reproductive system of male moths at different day-age and mating status were observed by dissection,and the classification standard was established.Male moths trapped by sex pheromone in field was dissected to analyze the mated status according to the classification standard above.The results showed that testis volume was decreased with the increase of day-age,and the mated testis became shrank and flattened in the later stage of development.But there was no change in the genital contents in primary segment(PS)of single ductus ejaculatorius with the increase of day-age.According to color and compactness of the genital contents in the end of PS,mated status were classified into five grades(Ⅰ-Ⅴ).The genital contents of unmated male adults were brown or dark brown(gradeⅤ),with high uniformity.The genital contents were completely excreted and became transparent after mating(gradeⅠ),and then it began to backfill(gradeⅡto gradeⅣ).There were 921 male moths were trapped in the field by sex pheromone traps,among which the number of mated males(gradeⅠto gradeⅣ)was 647 individuals.There were no significant differences in mated status of the male moth traped by different sex pheromone traps.This study provides a reference for the prediction of S.frugiperda and evaluation of the actual control effect of sex pheromone traps.

关 键 词：草地贪夜蛾 雄蛾 生殖结构 交配状态 精巢 性诱剂 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]