一种测试鳞翅目幼虫嗅觉行为的高效选择装置  

A selection device with high efficacy for detecting olfactory behavior of lepidopteran larvae

作　　者：俞佳宁 束长龙[1] 田小燕[1,2] 张杰 YU Jianing;SHU Changlong;TIAN Xiaoyan;ZHANG Jie(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;Shanxi Normal University, Linfen 041099, China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]山西师范大学,临汾041099

出　　处：《植物保护》2022年第3期199-203,共5页Plant Protection

基　　金：中国农业科学院创新工程专项所级重点任务(ZBSJZD03)。

摘　　要：昆虫的寄主定位、产卵选择、聚集、求偶等行为受到来自昆虫周围环境的挥发性气味化合物的影响和调控。研究挥发物对昆虫行为的影响作用主要采用Y形嗅觉仪等行为检测装置,而研究挥发物对鳞翅目幼虫的影响作用却缺乏专业、高效的装置。本研究设计制作了研究鳞翅目幼虫行为的高效选择装置,适用于高效筛选对鳞翅目幼虫具有引诱、驱避等作用的挥发物。小菜蛾3龄幼虫的行为选择测试结果表明,对小菜蛾幼虫引诱最强的植物为大白菜,其次是油菜和甘蓝;花生叶片对小菜蛾幼虫没有作用;而番茄叶片对小菜蛾幼虫则具有驱避作用。由此说明该装置可以有效测定小菜蛾幼虫对不同挥发物的反应,将为挥发物对昆虫行为的影响研究、进而筛选新的化合物来控制虫害提供新的技术支持。The behaviors of insect including host location,oviposition selection,aggregation and courtship are all regulated by the volatile compounds from the environment.The Y-tube olfactometer and other olfactometers are mainly used to study the effects of volatiles on the behavior of insects,but few professional and efficient devices for studying the effects of volatiles on lepidopteran larvae are available.In this study,an efficient selection device for studying the behavior of lepidopteran larvae was designed,which is suitable for efficient screening of volatile compounds with attractant and repellent effects on lepidopteran larvae.The results of behavioral selection test using the third-instar larvae of the diamondback moth Plutella xylostella showed that Chinese cabbage had the strongest attraction to the larvae in all of the samples we tested,followed by rape and cabbage.Meanwhile,peanut leaves showed no effect on diamondback moth larvae,while tomato leaves had a repellent effect on diamondback moth larvae.These results indicated that this device could effectively measure and distinguish the behavioral response of diamondback moth larvae to different volatiles.This device provides a new technical support for studying the effects of volatiles on insect behaviors and screening new compounds to control insect pests.

关 键 词：小菜蛾 选择装置 嗅觉 挥发物 

分 类 号：Q968.1[生物学—昆虫学]