BET蛋白抑制剂JQ-1对系统性红斑狼疮患者外周血B细胞分化的调控作用  

BET protein inhibitor JQ-1 regulates the differentiation of peripheral blood B cells in systemic lupus erythematosus

作　　者：李昊[1] 曾珊[1] 邱茜[1] 肖游君[1] 徐思琪 王竞男[1] 石茂华 许韩师[1] 梁柳琴[1] LI Hao;ZENG Shan;QIU Qian;XIAO Youjun;XU Siqi;WANG Jingnan;SHI Maohua;XU Hanshi;LIANG Liuqin(Department of Rheumatology and Immunology,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院风湿免疫科,广州510080

出　　处：《实用医学杂志》2022年第8期952-957,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81701611);广州市民生科技攻关计划(编号:201803010042)。

摘　　要：目的探讨BET蛋白抑制剂JQ-1对系统性红斑狼疮(SLE)患者外周血B细胞分化中的作用及机制。方法收集SLE患者外周血,分离纯化B细胞进行实验;体外培养SLE患者外周血B细胞;应用流式细胞术检测SLE患者外周血B细胞凋亡、存活及增殖的比例,检测外周血B细胞各亚群细胞的比例和B细胞体外诱导分化为浆母细胞的比例。结果与对照组相比,BET蛋白抑制剂JQ1对SLE外周血B细胞早期凋亡、存活及增殖无影响,但可显著下调浆母细胞、浆细胞亚群的比例,使浆母细胞体外诱导分化成浆细胞的比例减少。结论BRD4蛋白参与调控SLE患者外周血浆母细胞分化成浆细胞过程,JQ-1可能是SLE的潜在治疗药物。Objective To investigate the effect of BET protein inhibitor JQ-1 on the differentiation of peripheral blood B cells in systemic lupus erythematosus(SLE).Methods We collected the peripheral blood from 46 SLE patients,from which B cells were isolated,purified and cultured in vitro.Flow cytometry was used to detect the proportion of apoptosis,survival and proliferation of peripheral blood B cells,the proportion of subpopulation cells and the proportion of B cells differentiation into plasmablasts in vitro.Results Compared with the control group,the BET protein inhibitor JQ1 had little effect on the early apoptosis,survival and proliferation of the peripheral blood B cells,but it significantly reduced the rates of plasmablasts and plasma cells so as to reduce the proportion of B cells differentiation into plasmablasts in vitro.Conclusion BET protein inhibitor JQ-1 can regulate the differentiation of peripheral plasma blasts into plasma cells in SLE patients,indicating that it may have potential therapeutic effect on SLE.

关 键 词：系统性红斑狼疮 B细胞 BRD4蛋白 JQ-1 

分 类 号：R593.241[医药卫生—内科学]