高产β-甘露聚糖酶黑曲霉菌株的分离与鉴定  

Isolation and identification of high-yieldβ-mannanase Aspergillus niger

作　　者：王佰涛 刘德海[1] 陈晓飞[1] 刁文涛[2] 李珊珊[1] 雷高 WANG Baitao;LIU Dehai;CHEN Xiaofei;DIAO Wentao;LI Shanshan;LEI Gao(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou 450008,China;Henan Key Laboratory of Microbial Engineering,Zhengzhou 450008,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008 [2]河南省微生物工程重点实验室,河南郑州450008

出　　处：《中国酿造》2022年第5期101-105,共5页China Brewing

基　　金：中原科技创新领军人才项目(214200510011)。

摘　　要：该研究以采集的河南南阳魔芋种植土壤样品为试验材料,通过富集培养、测定产β-甘露聚糖酶酶活力,分离筛选高产β-甘露聚糖酶的菌株,并通过形态观察、内转录间隔区(ITS)基因和β-微管蛋白基因BenA序列分析对其进行菌种鉴定。结果表明,筛选得到一株高产β-甘露聚糖酶真菌菌株HKS016,其β-甘露聚糖酶酶活力为2.67 U/mL,该菌株被鉴定为黑曲霉(Aspergillus niger),保藏于中国普通微生物学菌种保藏中心(CGMCC),保藏编号为CGMCC No.22413。In this study,using soil samples collected from konjac cultivation in Nanyang,Henan province as test materials,the high-yield β-mannanase strains were isolated and screened by enrichment culture and determination of β-mannanase activity,and the strains were identified by morphological observation,internal transcribed spacer(ITS)gene and β-tubulin gene BenA sequence analysis.The results showed that a fungal strain HKS016 with high-yieldβ-mannanase was screened,and its β-mannanase activity was 2.67 U/ml.The strain was identified as Aspergillus niger and stored in the the China General Microbiological Culture Collection Center(CGMCC),numbered as CGMCC No.22413.

关 键 词：Β-甘露聚糖酶 分离 鉴定 黑曲霉 内转录间隔区 β-微管蛋白基因 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]