大鼠腹壁浅动脉皮瓣游离移植至腋窝模型的构建技巧  

Establishment of the groin flap based on the superficial epigastric artery into axillary fossa in rats

作　　者：吴东方[1] 刘东红 方芳 荆幸 陈绍锋 庄跃宏[3] Wu Dongfang;Liu Donghong;Fang Fang;Jing Xing;Chen Shaofeng;Zhuang Yuehong(College of laboratory Medicine(College of Life Sciences),Wenzhou Medical University,Wenzhou 325035,Zhejiang Province,China;Second Affiliated Hospital,Shandong First Medical University,Tai'an 271000,Shandong Province,China;Department of Human Anatomy,School of Basic Medicine,Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]温州医科大学检验医学院(生命科学学院),浙江温州325035 [2]山东第一医科大学第二附属医院,山东泰安271000 [3]福建医科大学基础医学院人体解剖学系,福州350122

出　　处：《中国临床解剖学杂志》2022年第3期271-276,共6页Chinese Journal of Clinical Anatomy

基　　金：浙江省医药卫生科技计划项目(2019KY447)。

摘　　要：目的推介将大鼠腹壁浅动脉皮瓣游离移植至腋窝的具体操作步骤。方法选取体重420~450 g的清洁级SD雄性大鼠20只,麻醉成功后备皮,设计以腹壁浅血管为蒂的4 cm×2 cm腹股沟皮瓣,游离移植至腋窝,以胸外侧血管为受区血管,重点观察:股血管及胸外侧血管的起源、走行与分支,测量其外径和长度;大鼠生存状态;术后第7 d皮瓣存活情况。结果股动、静脉的管径分别为(0.72±0.08)mm及(1.29±0.17)mm,胸外侧动、静脉的管径分别为(0.59±0.18)mm及(1.04±0.12)mm,两者适合做端端吻合。术后有2只大鼠死亡,另有3只大鼠皮瓣完全坏死,皮瓣存活率达75%。由于股动脉的破坏,患肢术后会出现跛行,1周后,随着血供的代偿,大鼠的步态逐渐恢复正常。结论大鼠腹壁浅动脉游离皮瓣是超级显微外科的基础研究与临床前操作练习的有效模型之一。Objective To describe the procedure of transfer of the groin flap based on the superficial epigastric artery into the axillary fossa in rats.Materials Twenty clean SD male rats weighing 420~450 g were depilated after successful anesthesia.A free groin flap model with a size of 4cm×2cm was established based on the superficial blood vessel of the abdominal wall was transplanted to the axillary fossa with the lateral thoracic vessels used as the recipient vessels.The main observations were as follows:①the origin,course and branches of femoral vessels and lateral thoracic vessels were observed,and their outer diameter and length were measured;②survival state of experimental animals;③The survival of the flap on the 7th day after operation.Results The diameter of the femoral artery and vein was(0.72±0.08)mm and(1.29±0.17)mm respectively,and that of the lateral thoracic artery and vein was(0.59±0.18)mm and(1.04±0.12)mm respectively.Two rats failed to recover from anesthesia after operation,and 3 rats had complete necrosis of skin flap,resulting in a flap survival rate of 75%.Due to removal of the femoral artery,claudication of the affected limb could occur after surgery.One week later,with compensation of the blood supply,the gait of rats gradually returned to normal.Conclusions The free flap based on the superficial epigastric artery in rats is very suitable for junior doctors and researchers who want to engage in the clinical work and the basic research relating to supramicrosurgery.

关 键 词：游离皮瓣 腹壁浅动脉 胸外侧动脉 血管吻合 

分 类 号：R331[医药卫生—人体生理学] R332[医药卫生—基础医学]