基于微卫星引物鉴别不同地理种群玉米螟赤眼蜂和螟黄赤眼蜂  

Identification of different geographical populations of Trichogramma ostriniae and Trichogramma chilonis based on microsatellite primers

作　　者：安仕博 陈旭 戴鹏[1] 侯洋旸 臧连生[1,2] AN Shi-Bo;CHEN Xu;DAI Peng;HOU Yang-Yang;ZANG Lian-Sheng(Institute of Biological Control,Jilin Agricultural University,Changchun 130118,China;Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang 550025,China)

机构地区：[1]吉林农业大学生物防治研究所,长春130118 [2]贵州大学绿色农药与农业生物工程教育部重点实验室,贵阳550025

出　　处：《环境昆虫学报》2022年第2期414-421,共8页Journal of Environmental Entomology

基　　金：国家重点研发计划(2019YFD0300105);国家自然科学基金(31901946)。

摘　　要：不同地理种群的赤眼蜂在遗传、生理和生态适应性等方面均表现出不同程度的分化。为探究不同地理种群的玉米螟赤眼蜂Trichogramma ostriniae和螟黄赤眼蜂Trichogramma chilonis在基因型上的差异并进行准确鉴定,本研究筛选了可用以区分不同地理种群玉米螟赤眼蜂和螟黄赤眼蜂的微卫星引物。结果表明:从已报道的10对微卫星引物中,筛选出2对引物(序列号:KT834825,KT834827)可以区分玉米螟赤眼蜂中的黑龙江地理种群与吉林和辽宁两个地理种群;并筛选出2对引物(序列号:KT834822,KT834825)可以区分黑龙江、贵州和广东3个不同地理种群的螟黄赤眼蜂。该结果进一步证实赤眼蜂不同地理种群间可能存在明显的基因型差异,研究结果为精准鉴别玉米螟赤眼蜂和螟黄赤眼蜂不同地理种群,以及进一步探寻优势种群的高效繁育与应用技术奠定了理论基础。Different geographical populations of Trichogramma parasitoids have differentiation in genetic,physiological and ecological adaptability aspects.In order to explore the genotypic differences and accurate identification of different geographical populations of Trichogramma ostriniae and Trichogramma chilonis,microsatellite primers were screened to distinguish them.The results showed that:Two pairs of primers(sequence number:KT834825,KT834827) were selected from 10 pairs of microsatellite primers,which could distinguish Heilongjiang population and Jilin(or Liaoning) populations of T.ostriniae;and two pairs of primers(sequence number:KT834822,KT834825) could distinguish the three geographical population of T.chilonis from Heilongjiang,Guizhou and Guangdong Provinces.The results further confirmed that there may be significant genotypic differences among different geographical populations of Trichogramma.The results provided a theoretical foundation for accurate identification of different geographical populations of T.ostriniae and T.chilonis,and further exploration of efficient breeding and application technology of dominant populations.

关 键 词：玉米螟赤眼蜂 螟黄赤眼蜂 微卫星标记 地理种群 引物筛选 

分 类 号：Q968.1[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]