Shoot rot of Zizania latifolia and the first record of its pathogen Pantoea ananatis in China  

作　　者：Zilan XIAO Jianping DENG Xiaojun ZHOU Liyan ZHU Xiaochan HE Jingwu ZHENG Deping GUO Jingze ZHANG 

机构地区：[1]Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China [2]Plant Protection and Plant Inspection Station of Jinyun County,Jinyun 321401,China [3]Jinhua Academy of Agricultural Sciences,Jinhua 321017,China [4]Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2022年第4期328-338,共11页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Key Project of National Natural Science Foundation of China(NSFC)Regional Innovation and Development Joint Foundation(No.U20A2043);the NSFC(No.31501342)。

摘　　要：The aquatic grass Zizania latifolia grows symbiotically with the fungus Ustilago esculenta producing swollen structures called Jiaobai,widely cultivated in China.A new disease of Z.latifolia was found in Zhejiang Province,China.Initial lesions appeared on the leaf sheaths or sometimes on the leaves near the leaf sheaths.The lesions extended along the axis of the leaf shoots and formed long brown to dark brown streaks from the leaf sheath to the leaf,causing sheath rot and death of entire leaves on young plants.The pathogen was isolated and identified as the bacterium Pantoea ananatis,based on 16 S ribosomal RNA(r RNA)gene sequencing,multilocus sequence analysis(atp D(β-subunit of ATP synthase F1),gyr B(DNA gyrase subunit B),inf B(translation initiation factor 2),and rpo B(β-subunit of RNA polymerase)genes),and pathogenicity tests.Ultrastructural observations using scanning electron microscopy revealed that the bacterial cells colonized the vascular tissues in leaf sheaths,forming biofilms on the inner surface of vessel walls,and extended between vessel elements via the perforated plates.To achieve efficient detection and diagnosis of P.ananatis,species-specific primer pairs were designed and validated by testing closely related and unrelated species and diseased tissues of Z.latifolia.This is the first report of bacterial sheath rot disease of Z.latifolia caused by P.ananatis in China.

关 键 词：Zizania latifolia PHYLOGENY Pantoea ananatis Multilocus analysis Scanning electron microscopy Species-specific primers 

分 类 号：S436.45[农业科学—农业昆虫与害虫防治]