基于转录组测序筛选高盐培养下双峰驼肾皮质细胞差异表达基因  

Transcriptome Sequencing Screened Differentially Expressed Genes in Kidney Cortex Cells of Bactrian Camel Under High Salt Condition

作　　者：陈丽慧 萨日娜 宝山 敖特根巴雅尔 朝鲁门格日乐 黄海峰 孙波 额尔敦木图[1] 杨彬[1] CHEN Li-hui;Sarna;BAO Shan;Aotgenbayar;Cholmengerl;HUANG Hai-feng;SUN Bo;Erdemtu;YANG Bin(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Alxa League Institute of Animal Health Supervision,Bayanhot 750306,China;Inner Mongolia Bayannur City Institute of Agriculture and Animal Husbandry,Bayannur 015000,China;Animal Disease Prevention and Control Center of Agriculture and Animal Husbandry Bureau of Otg Banner,Ordos 016100,China;Comprehensive Administrative Law Enforcement Bureau of Ecological Management in Agricultural and Pastoral Areas of Alxa Left Banner,Bayanhot 750300,China;Comprehensive Security and Technology Promotion Center of Baronbel Town of Alxa Left Banner,Bayanhot 750300,China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]阿拉善盟动物卫生监督所,内蒙古巴彦浩特750306 [3]内蒙古自治区巴彦淖尔市农牧业科学研究所,内蒙古巴彦淖尔015000 [4]鄂托克旗农牧局动物疫病预防控制中心,内蒙古鄂尔多斯016100 [5]阿拉善左旗农牧区生态管理综合行政执法局,内蒙古巴彦浩特750300 [6]内蒙古阿拉善左旗巴润别立镇综合保障和技术推广中心,内蒙古巴彦浩特750300

出　　处：《中国兽医杂志》2022年第3期1-8,14,共9页Chinese Journal of Veterinary Medicine

基　　金：内蒙古农业大学高层次人才科研启动项目(NDYB2018-27);内蒙古自治区科技计划项目(201502069)。

摘　　要：为探讨双峰驼的高盐适应机制并筛选出受高盐调控的基因,本试验采用RNA-Seq技术对双峰驼肾皮质细胞进行转录组测序分析。试验分为2个组,等渗培养基处理的肾皮质细胞为对照组(Control),高渗培养基处理的肾皮质细胞为高渗组(HS),进行转录组测序,从而筛选出一系列差异表达基因(DEGs),并用GO富集和KEGG富集分析对DEGs进行基因功能和途径的注释。结果显示,在高盐环境下双峰驼肾皮质细胞中共有4854个显著DEGs;GO富集分析显示,DEGs显著富集在G蛋白偶联受体活性、受体结合、核酸结合转录因子活性、质膜、离子通道的活动和免疫反应等功能中;KEGG通路富集显示,DEGs显著富集在信号传导、辅助因子和维生素代谢、信号分子相互作用、运输和分解代谢、免疫系统等通路中;采用实时荧光定量PCR技术随机检测了参与高盐代谢的3个差异表达基因,其表达趋势与RNA-Seq一致,可以验证RNA-Seq技术的准确性。因此,双峰驼肾皮质细胞的高盐耐受性可能与这些途径和通路中重要基因的调控有关。本试验结果将为进一步揭示双峰驼耐盐性的分子调控机制提供重要的参考依据。This study utilized RNA-Seq technology to analyze the transcriptional expression of bactrian camel renal cortical cells under the high salt conditions.Transcriptome analyses were carried out on renal cortical cells in hypertonic stress group and isotonic control group.A series of differential expression genes(DEGs) were screened,and the gene function and pathway of DEGs were annotated by Gene Ontology enrichment and KEGG enrichment analysis.The results showed that among 4 854 DEGs identified in renal cortical cells of bactrian camel under high salt condition,Gene Ontology enrichment analysis revealed significant enrichment in G-protein coupled receptor activity,receptor binding,nucleic acid binding transcription factor activity,plasma membrane,ion channel activity,immune response and other pathways;KEGG pathway enrichment indicated that DEGs were significantly enriched in signal transduction,metabolism of cofactors and vitamins,signaling molecules and interaction,transport and catabolism,immune system and other pathways;qRT-PCR examination of three differentially expressed genes involved in high salt metabolism confirmed the accuracy of RNA-Seq analysis.Thus,the high salt tolerance of renal cortex cells in bactrian camel appears to relate to the regulation of several pathways and associated genes.These data provide an important references for further elucidation on the molecular regulation mechanism of salt tolerance in bactrian camel.

关 键 词：高盐 双峰驼 肾皮质细胞 转录组测序 差异表达基因 

分 类 号：S852.1[农业科学—基础兽医学]