牛源致脑炎大肠杆菌FimD、PilN蛋白的生物信息学分析和优势B细胞表位预测  

Bioinformatics Analysis of FimD and PilN Proteins and Prediction of Dominant B Cell Epitopes in Escherichia coli Causing Bovine Encephalitis

作　　者：海永慧 钦倩 李蓓蓓[1] 任静静[1] 马勋[1] 蒋建军[1] 王鹏雁[1] HAI Yong-hui;Qin Qian;LI Bei-bei;REN Jing-jing;MA Xun;JIANG Jian-jun;WANG Peng-yan(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国兽医杂志》2022年第3期9-14,F0003,F0002,共8页Chinese Journal of Veterinary Medicine

基　　金：NSFC-新疆联合基金(U1803109)。

摘　　要：为了筛选出牛源致脑炎大肠杆菌S9922 FimD、PilN蛋白的优势B细胞表位,本试验采用生物信息学分析工具ProtParam、TMHMM、NetPhos、DNASTAR软件、SOPMA、SWISS-MODEL、BepiPred、Immunomedicine Group等对FimD、PilN蛋白进行理化性质、跨膜区、信号肽、糖基化位点、磷酸化位点、二级结构、三级结构和B细胞表位预测,并进行综合分析,最终得出优势B细胞表位。结果显示,FimD、PilN蛋白都为亲水、稳定蛋白,无规则卷曲在二级结构中所占比例最高;FimD蛋白共有48个磷酸化位点,不存在跨膜区和信号肽;PilN蛋白共有85个磷酸化位点,PilN蛋白在1~50位氨基酸上可能出现跨膜区,26~27位氨基酸上出现信号肽;多种参数综合分析最终得到潜在的优势B细胞表位,FimD蛋白:7~18、144~152、307~315、365~373;PilN蛋白:77~86、138~149、516~524。通过对FimD、PilN蛋白进行综合分析,最终得出潜在的优势B细胞表位,为牛源致脑炎大肠杆菌多表位疫苗的进一步研究提供参考依据。In order to identify the dominant B cell epitopes in FimD and PilN proteins from bovineEscherichia coliS9922,this study employed bioinformatics analysis tools such as ProtParam,TMHMM,NetPhos,DNASTAR,SOPMA,SWISS-MODEL,BepiPred and Immunomedicine Group to predict the physicochemical properties,transmembrane regions,signal peptides,glycosylation sites,phosphorylation sites,secondary structures,tertiary structures and B cell epitopes in FimD and PilN proteins.The results showed that both FimD and PilN were hydrophilic and stable proteins,and irregular curling accounted for the highest proportion in the secondary structure;48 phosphorylation sites were present in FimD protein,and no transmembrane region and signal peptide in FimD protein were found;85 phosphorylation sites existed in PilN protein,and transmembrane region might appear in PilN protein at 1-50amino acid position,and signal peptide appeared at 26-27 amino acid position.Comprehensive analysis of various parameters identified dominant B cell epitopes in FimD protein at 7-18,144-152,307-315,365-373,and in PilN protein at 77-86,138-149,516-524.Thus,comprehensive analysis of FimD and PilN proteins and identification of the dominant B cell epitopes laid the foundation for further development of multi-epitope vaccine againstEscherichia colicausing bovine encephalitis.

关 键 词：牛源致脑炎大肠杆菌 FimD蛋白 PilN蛋白 生物信息学分析 B细胞抗原表位 

分 类 号：S855.1[农业科学—临床兽医学]