金堂黑山羊传染性脓疱的病理组织学观察和病毒分子检测  

Histopathological Observation and Virus Molecular Detection of Contagious Ecthyma in Jintang Black Goats

作　　者：向益 豆朋朋 王利[1] 魏勇[2] 俄木曲者[2] 苗斌 陈勇 XIANG Yi;DOU Peng-peng;WANG Li;WEI Yong;EMU Quizhe;MIAO Bin;CHEN Yong(College of Animal&Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Animal Science Academy of Sichuan Province,Chengdu 610066,China;Nanjiang Yellow Goat Scientific Research Institute,Nanjiang 635600,China)

机构地区：[1]西南民族大学畜牧兽医学院,四川成都610041 [2]四川省畜牧科学研究院,四川成都610066 [3]四川省巴中市南江黄羊科学研究所,四川南江635600

出　　处：《中国兽医杂志》2022年第3期37-41,共5页Chinese Journal of Veterinary Medicine

基　　金：四川省肉羊创新团队防疫岗位(SCCXTD-2020-14);四川省科技厅项目(2021YFN007,2021YFN003)。

摘　　要：为确定四川成都某羊场黑山羊发病的病原并探讨其病理变化特点,本试验观察了患病羊的临床症状及剖检变化,制备了其唇、舌、心脏、肝脏、脾脏、肺脏的病理组织切片,观察其病理变化;并对患病羊的口唇病料样品进行羊传染性脓疱病毒(CEV)F1L基因的PCR检测。剖检结果显示,患病金堂黑山羊口、唇出现大小不等的丘疹、溃疡和脓疱;心包积液、出血;肾脏、脾脏肿大、淤血。病理组织学检查结果显示,病变唇、舌部表皮棘细胞大量增生,棘细胞空泡变性、网状变性、气球样变性,胞浆内可见嗜酸性病毒包涵体;心肌细胞排列紊乱,心肌纤维肿胀,成纤维细胞增多;肝细胞水肿,空泡变性;脾淋巴鞘增厚,血窦扩张、充血;肺泡扩张、破裂,肺泡壁变薄。PCR检测结果显示,患病羊口唇病料样品扩增出大小为230 bp的F1L基因片段;测序后BLAST比对显示,该基因序列与GenBank中登录的羊传染性脓疱病毒F1L基因序列同源性达99%。上述结果表明,该羊场此次发病由CEV感染引起,且CEV主要侵害金堂黑山羊唇、舌部的上皮组织,并且可造成心肌细胞肿胀、肝细胞变性、脾窦充血、肺泡扩张等病理损伤。本试验结果为CEV的诊断与治疗提供了科学依据。In order to verify the cause of black goat disease on a goat farm in Chengdu of Sichuan province and determine its pathological characteristics,this study conducted detailed assessment of clinical symptoms and autopsy changes in disease goats;observed histopathological changes in the lip,tongue,heart,liver,spleen,lung pathological tissue slice;and collected oral tissue samples for PCR detection of contagious ecthyma virus(CEV) F1L gene.Necropsy results showed that pimples,ulcers and pustules of different sizes appeared in the mouth and lips of diseased Jintang black goats,along with pericardial effusion and hemorrhage,swollen kidney,spleen and congestion.Histopathological examination showed acanthocyte proliferation in the epidermal spine of the affected lip and tongue,vacuolar degeneration,reticular degeneration and balloon-like degeneration of the acanthocytes,and eosinophilic virus inclusions in the cytoplasm;disordered arrangement of myocardial cells,swollen myocardial fibers and increasing fibroblasts;hepatocyte edema,vacuolar degeneration;thickened spleen lymphatic sheath,dilated blood sinus,congestion;alveolar expansion,rupture,and alveolar wall thinning.PCR results showed that the size of F1L gene product was 230 bp,which had a 99 % homology with F1L gene of CEV registered in GenBank.These results indicated that the disease in Jintang black goats was likely due to CEV infection,which mainly affected the epithelial tissues of the lip and tongue and caused myocardial cell swelling,hepatocyte degeneration,splenic sinus congestion,alveolar dilation and other pathological injuries.This study provides valuable experimental insights for the diagnosis and treatment of CEV.

关 键 词：金堂黑山羊 羊传染性脓疱病毒 组织病理学 剖检 

分 类 号：S852.3[农业科学—基础兽医学]