基于ITS2条形码鉴定粤产竹根薯及其易混淆品  

Identification of Maranta arundinacea L. and its Analogues from Guangdong based on ITS2 Barcode

作　　者：陈超志 林勤 程芷琰 李龙明 罗肖华 蔡宇忆 叶永浩 CHEN Chaozhi;LIN Qin;CHENG Zhiyan;LI Longming;LUO Xiaohua;CAI Yuyi;YE Yonghao(Dongguan Polytechnic,Dongguan,Guangdong 523000,China;Guangdong Maoming Health Vocational College,Maoming,Guangdong 525000,China;Zhuhai Body Revival Healthy Technology Co.,Ltd.,Zhuhai,Guangdong 519000,China;Zhuhai Resproly Pharmaceutical Technology Co.,Ltd.,Zhuhai,Guangdong 519000,China)

机构地区：[1]东莞职业技术学院,广东东莞523000 [2]广东茂名健康职业学院,广东茂名525000 [3]珠海宝德润生健康科技有限公司,广东珠海519000 [4]珠海瑞思普利医药科技有限公司,广东珠海519000

出　　处：《今日药学》2022年第2期120-123,共4页Pharmacy Today

基　　金：东莞职业技术学院校级基金(2019d01);广东省科技创新战略和乡村振兴战略专项资金(“大专项+任务清单”)2021S0056。

摘　　要：目的 采用DNA条形码分子鉴定技术鉴定粤产竹根薯(又名竹芋)Marantak arundinacea L.与及其易混淆品(包括飞羽竹芋、苹果竹芋和双线竹芋),以确保竹根薯药材质量。方法 收集药材样品,对其ITS2序列进行PCR扩增并正向测序,所得序列用Codon Code Aligner软件校对拼接后,利用MEGA 6.05对序列进行分析比对,计算种内、种间遗传距离,利用邻接法(NJ)构建系统聚类树。结果 竹根薯种内遗传距离明显小于它与其同属的种间遗传距离,基于ITS2序列建立了NJ树,能准确将竹根薯与其混淆品区分。结论 基于ITS2序列可以科学准确地对竹根薯及其易混淆品进行鉴定,为竹根薯的生药鉴别提供新的科学依据。OBJECTIVE To distinguish Marantae Rhizoma and its analogues from Guangdong by DNA barcode molecular identification technology,to ensure the quality.METHODS The samples were collected and their ITS2 sequences were amplified by PCR and sequenced forward.The sequences were collated and spliced with Codon Code Aligner software.MEGA 6.05 was used to analyze and compare the sequences,calculate intra-specific and inter-specific genetic distances,and construct a cluster tree by adjacency method.RESULTS The intra-specific genetic distances were smaller than inter-specific ones in ITS2 regions of Maranta arundinacea L.The NJ tree was established based on ITS2 sequence,which could accurately distinguish Maranta arundinacea L.and its analogues.CONCLUSION The ITS2 sequence can be used to identify Maranta arundinacea L.and its analogues scientifically and accurately,providing a new scientific basis for crude drug identification of Tuber bambusa.

关 键 词：竹根薯 混淆品 鉴别 DNA条形码 

分 类 号：R931.5[医药卫生—生药学]