2020年陕西省布鲁氏菌MLVA分型研究及流行病学分析  

MLVA typing and epidemiological analysis of Brucella in Shaanxi Province,2020

作　　者：聂守民 罗波艳 孙养信[1] 范锁平[1] 常文辉[1] 孙杰 安翠红[1] Nie Shoumin;Luo Boyan;Sun Yangxin;Fan Suoping;Chang Wenhui;Sun Jie;An Cuihong(Institute for Plague and Brucellosis Prevention and Control,Shaanxi Provincial Center for Disease Control and Prevention,Xi'an 710054,China;Department for Endemic Disease Prevention,Chengcheng County Center for Disease Control and Prevention,Weinan 714000,China)

机构地区：[1]陕西省疾病预防控制中心鼠疫布病防控所,西安710054 [2]澄城县疾病预防控制中心地方病科,渭南714000

出　　处：《中华地方病学杂志》2022年第3期180-185,共6页Chinese Journal of Endemiology

基　　金：陕西省重点研发计划项目(2018SF-001)。

摘　　要：目的了解陕西省布鲁氏菌病发病情况及分离菌株或核酸的基因型,掌握其流行病学及分子遗传特征,为陕西省人间布鲁氏菌病的精准防控提供科学依据。方法登录中国疾病预防控制信息系统收集2020年陕西省人间布鲁氏菌病的发病数据,分析流行特征;应用细菌学及PCR方法对分离菌株或核酸进行鉴定,进一步采用多位点可变数目串联重复序列分析(MLVA)方法进行分子分型,利用BioNumerics(Version 7.6)软件对MLVA结果进行分析。结果2020年陕西省共报告人间布病1086例,发病率为2.80/10万,涉及86个县(区),流行高峰在3-9月(865例),男女性别比为2.68∶1.00(791∶295),30~69岁年龄段占79.74%(866例),农民占83.43%(906例)。36株分离菌株生物型鉴定结果显示,4株是羊种变异型,3株是羊种1型,29株是羊种3型。36株分离菌株及7份核酸经BCSP31-PCR均鉴定为布鲁氏菌属,经AMOS-PCR均鉴定为羊种布鲁氏菌。经MLVA-16分型,panel1结果显示有2种基因型:42型(1-5-3-13-2-2-3-2)、63型(1-5-3-13-2-3-3-2),panel2A结果显示均为4-41-8,panel2B结果显示具有高度的多变性;36株分离菌株及7份核酸分为33种基因型,其中27种基因型为单分离株,6种基因型为共享型。结论陕西省人间布鲁氏菌病防控形势严峻。MLVA-16是一种成熟的基因分型方法,确定了陕西省布鲁氏菌分离株或核酸存在多种基因型,可为人间布鲁氏菌病精准防控、暴发疫情分析及流行病学溯源提供科学依据。Objective To analyze the incidence of brucellosis and the genotypes of Brucella isolates or nucleic acids in Shaanxi Province,to get the epidemiological and molecular genetic characteristics,and to provide scientific basis for precise prevention and control of human brucellosis.Methods Log into the Chinese Disease Control and Prevention Information System,collect the incidence data of human brucellosis of Shaanxi Province in 2020,and analyze the epidemiological characteristics.Bacteriology and PCR methods were used to identify the isolates or nucleic acids,and multiple locus variable-number tandem repeat analysis(MLVA)was used for molecular typing.BioNumerics(Version 7.6)software was used to analyze the results of MLVA.Results In 2020,1086 cases of human brucellosis were reported in Shaanxi Province,the incidence rate was 2.80/100000,involving 86 counties(districts),the epidemic peak was from March to September(865 cases),male-to-female ratio was 2.68∶1.00(791∶295),79.74%(866 cases)in the age group of 30 to 69 years old,and 83.43%(906 cases)of the cases were farmers.Biotype identification of 36 isolates showed that 4 isolates were mutant Brucella melitensis,3 isolates were Brucella melitensis 1 and 29 isolates were Brucella melitensis 3.The 36 isolates and 7 nucleic acids were identified as Brucella by BCSP31-PCR and Brucella melitensis by AMOS-PCR.MLVA-16 genotyping,panel1 showed two genotypes:type 42(1-5-3-13-2-2-3-2)and type 63(1-5-3-13-2-3-3-2),panel2A showed 4-41-8 and panel2B showed high variability.Thirty-six isolates and 7 nucleic acids were divided into 33 genotypes,of which 27 genotypes were single isolates and 6 genotypes were shared.Conclusions The situation of human brucellosis prevention and control in Shaanxi Province is grim.MLVA-16 is a mature genotyping method,which determines the existence of multiple genotypes of Brucella isolates or nucleic acids in Shaanxi Province,which can provide scientific information for precise prevention and control of human brucellosis,outbreak analysis and epide

关 键 词：布鲁杆菌属 多位点可变数目串联重复序列分析 流行病学分析 

分 类 号：R516.7[医药卫生—内科学] R181.3[医药卫生—临床医学]