内生砖红镰刀菌对番茄生长和抗病的影响  

Effects of an endophytic Fusarium lateritium on growth and disease resistance of tomato

作　　者：肖青 李永杰 汪健康 查兴平 黄建明 何张江 康冀川[1] XIAO Qing;LI Yongjie;WANG Jiankang;ZHA Xingping;HUANG Jianming;HE Zhangjiang;KANG Jichuan(Engineering Research Center of Southwest Bio-Pharmaceutical Resources,Ministry of Education,Guizhou University,Guiyang 550025,China;College of Life Sciences,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学西南特色药用生物资源开发利用教育部工程研究中心,贵阳550025 [2]贵州大学生命科学学院,贵阳550025

出　　处：《华中农业大学学报》2022年第3期173-180,共8页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(32170019,32160667,31901947);贵州省国际合作项目(黔科合基础-ZK[2021]145);贵州大学引进人才基金项目(贵大人基合字[2019]71号)。

摘　　要：为探究内生真菌砖红镰刀菌在番茄生长及抗病过程中的作用,使用1×10^(7) CFU/mL砖红镰刀菌芽生孢子悬液浸根处理发芽的番茄,结果显示:处理50 d后番茄株高较对照组增加了1.15倍;叶绿素a、叶绿素b含量较对照组分别增加1.16倍和1.47倍;根系生物量较对照增加1.38倍;砖红镰刀菌处理组对番茄枯萎病的病情指数(45.16%)较对照组(74.15%)下降;浸根处理20 d时植物生长素合成关键基因SlYUC5、水杨酸合成关键基因SlICS1表达水平与未处理对照组相比无显著差异;处理30及40 d后,SlYUC5表达水平较对照组相比表达显著上升,SlICS1表达水平较对照组显著降低;茉莉酸合成途径关键基因SlLOXD在20 d时表达水平较对照组显著下调38.73%(P<0.01),而在30 d时其表达量与对照组无显著差异,40 d后,其表达量较对照组显著上调(2.33倍,P<0.01);试验组中植物抗性相关蛋白基因SlPR1a在20、30和40 d的表达量被显著上调了7.89倍、5.77倍和1.8倍(P<0.01)。进一步用GFP标记菌株进行荧光定殖观察并通过qPCR法计算其定殖率,14 h后在番茄根系观察到镰刀菌菌丝存在,并随处理时间延长定殖率逐渐上升。综上,推测砖红镰刀菌能够通过内生定殖于番茄根系介导植物激素相关基因表达从而影响植株的长生发育和抗病。The previous studies in authors’group indicated that an endophytic Fusarium lateritium from the Nothapodytes pittosporoides(Oliv.)plant may promote the growth and disease resistance of pota⁃to.In order to expand the application scope of this strain,tomato,which is also a Solanaceae crop,was used to study the effects of F.lateritium on the growth and disease resistance.The results showed that the plant height of tomato increased by 1.15 times compared with the control group after 50 days of root-soaking treatment with F.lateritium.The content of chlorophyll a and chlorophyll b were 1.16 times and 1.47 times higher than that of the control group.The root biomass of the treatment was 1.38 times higher than that of the control,indicating that F.lateritium promotes the growth of tomato.The results of bioassay showed that the fusarium wilt disease index of tomato(45.16%)in the treatment group with F.lateritium was about 28.99%lower than that in the control group(74.15%),indicating that F.lateritium enhances the resis⁃tance of tomato to fusarium wilt.The expression patterns of key genes in the auxin synthesis,salicylic acid synthesis and jasmonic acid pathway of tomato were analyzed after 20,30 and 40 days of treatment.The re⁃sults showed that the treatment affected the expression of key genes of plant hormones to different degrees.The GFP-labeled strain was further used for fluorescence colonization observation and the colonization rate was calculated by qPCR method.After 14 hours,the presence of F.hyphae was observed in the root system of tomato.The colonization rate of F.hyphae gradually increased with the extension of the treatment time within a certain period of time,indicating that F.lateritium can endogenously colonize the root system of to⁃mato.In conclusion,it is speculated that F.lateritium can mediate the expression of phytohormone-related genes through the endogenous colonization in roots of tomato,thereby affecting the plant growth and dis⁃ease resistance of tomato.

关 键 词：内生真菌 砖红镰刀菌 番茄 促生 抗病 

分 类 号：S641.2[农业科学—蔬菜学] Q949.32[农业科学—园艺学]