基于SSR标记分析蓝莓品种‘蓝美1号’自由授粉子代遗传多样性及群体遗传结构  

Analysis on genetic diversity and population genetic structure of open-pollinated progenies of Vaccinium corymbosum‘Lanmei 1’based on SSR marker

作　　者：王亮 韦继光[1] 葛春峰 於虹[1] 姜燕琴[1] 杨曙方 曾其龙[1] 田亮亮[1] WANG Liang;WEI Jiguang;GE Chunfeng;YU Hong;JIANG Yanqin;YANG Shufang;ZENG Qilong;TIAN Liangliang(Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;Zhejiang Lanmei Technology Co., Ltd., Shaoxing 312000, China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]浙江蓝美技术股份有限公司,浙江绍兴312000

出　　处：《植物资源与环境学报》2022年第3期35-43,共9页Journal of Plant Resources and Environment

基　　金：所企合作项目(‘蓝美1号’高效栽培技术与新品种选育)自主创新资金资助项目〔CX(21)3044〕。

摘　　要：基于SSR标记对蓝莓品种‘蓝美1号’(Vaccinium corymbosum‘Lanmei 1’)217个自由授粉子代和26个现有蓝莓(Vaccinium spp.)栽培品种的遗传多样性和群体遗传结构进行了分析。结果显示:243个供试材料中,23对引物共检测出113个等位基因。多态性信息含量指数(PIC)为0.5075~0.8330,均值为0.6866;所有引物的PIC值均在0.5以上,表明SSR引物的多态性较高。217个自由授粉子代的Nei’s遗传多样性指数和Shannon’s多态性信息指数的均值分别为0.7334和1.4394,自由授粉子代较现有栽培品种具有较高的遗传多样性水平。UPGMA聚类分析中,26个品种聚为一类;217个自由授粉子代的遗传相似系数为0.70~0.99,除少数自由授粉子代外,其余子代划分为3个类群。STRUCTURE分析将217个自由授粉子代划分为4个亚群,亚群内子代遗传背景相对单一。比较UPGMA聚类分析和STRUCTURE分析的结果,STRUCTURE分析的分群效果更佳,亚群内子代间遗传相似性高。综合分析结果表明:筛选出的23对引物具有高度多态性;‘蓝美1号’自由授粉子代的遗传多样性较高,同现有栽培品种亲缘关系较远,可为蓝莓品种的改良提供丰富的遗传资源。Genetic diversity and population genetic structure of 217 open-pollinated progenies of Vaccinium corymbosum‘Lanmei 1’and 26 existing cultivated cultivars of blueberry(Vaccinium spp.)were analyzed based on SSR marker.The results show that among 243 test materials,113 alleles are detected with 23 pairs of primers.The polymorphic information content index(PIC)is 0.5075-0.8330,with an average of 0.6866;the PIC values of all primers are greater than 0.5,indicating the polymorphisms of SSR primers are relatively high.The averages of Nei’s genetic diversity index and Shannon’s polymorphic information index of 217 open-pollinated progenies are 0.7334 and 1.4394 respectively,and the genetic diversity levels of open-pollinated progenies are relatively higher than those of existing cultivated cultivars.In the UPGMA cluster analysis,26 cultivars are clustered into one category;the genetic similarity coefficient of 217 open-pollinated progenies are 0.70-0.99,and expect for a few open-pollinated progenies,the other progenies can be divided into three groups.The STRUCTURE analysis divides 217 open-pollinated progenies into four subgroups,and the genetic background of progenies within subgroups are relatively simple.Comparing the results of UPGMA cluster analysis and STRUCTURE analysis,the grouping effect of STRUCTURE analysis is better,and the genetic similarity among progenies within subgroups is high.The comprehensive analysis result shows that 23 pairs of selected primers are highly polymorphic;the genetic diversity of open-pollinated progenies of‘Lanmei 1’is relatively high and they have relatively far genetic relationships with existing cultivated cultivars,which can provide rich genetic resources for improvement of blueberry(Vaccinium spp.)cultivars.

关 键 词：‘蓝美1号’ 蓝莓 SSR标记 遗传多样性 群体遗传结构 

分 类 号：Q949.9[生物学—植物学] S663.9[农业科学—果树学]