NaCl胁迫下不同耐盐型结缕草根系Ca^(2+)和H^(+)流动及Ca^(2+)分布特征  

Ca^(2+)and H^(+)flow and Ca^(2+)distribution characteristics in roots of Zoysia japonica with different salt tolerances under NaCl stress

作　　者：叶刚 杨静[2] 李晓慧 郝东利 沈泽宇 郑荣佳 陈静波[1] 郭海林[1] YE Gang;YANG Jing;LI Xiaohui;HAO Dongli;SHEN Zeyu;ZHENG Rongjia;CHEN Jingbo;GUO Hailin(Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China;Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]南京林业大学,江苏南京210037

出　　处：《植物资源与环境学报》2022年第3期75-84,共10页Journal of Plant Resources and Environment

基　　金：国家自然科学基金面上项目(31572155);中央财政林业科技推广示范资金项目(苏[2021]TG02)。

摘　　要：以耐盐结缕草(Zoysia japonica Steud.)Z011和Z015和敏盐结缕草Z004和Z103为试验材料,利用非损伤微测技术和X射线能谱仪对结缕草在NaCl胁迫(200 mmol·L-1 NaCl)下不同根区Ca^(2+)、H+流的变化以及Ca^(2+)在根系的分布情况进行了分析。结果表明:在对照条件下,4个结缕草材料根尖分生区、伸长区和成熟区Ca^(2+)以外排为主,其中分生区的平均Ca^(2+)流速明显高于伸长区和成熟区,且耐盐结缕草分生区的平均Ca^(2+)流速明显高于敏盐结缕草;NaCl胁迫后,总体上看,耐盐结缕草3个根区的Ca^(2+)由外排变为内流,而敏盐结缕草仅根尖成熟区的Ca^(2+)由外排变为内流,耐盐结缕草较敏盐结缕草有更多的Ca^(2+)内流参与离子调控。耐盐结缕草Z011与其他3个材料的H+流有明显区别,在对照条件下,Z0113个根区的H+均为内流,而其他3个材料3个根区的H+为微弱内流或外排,且其平均H+流速明显低于Z011;NaCl胁迫后,Z0113个根区H+内流明显减弱,而其他3个材料3个根区H+的内流或外排也减弱,但差异不明显。扫描结果显示:总体上看,NaCl胁迫后,4个结缕草材料根系表皮、皮层和中柱中Ca^(2+)相对含量显著降低,且随着处理时间的延长表现为先降低再升高的趋势;同一材料根系表皮、皮层和中柱间的Ca^(2+)相对含量存在差异,但区隔作用不明显。综合分析结果表明:耐盐结缕草有更强的Ca^(2+)调控能力,分生区为Ca^(2+)参与NaCl胁迫下离子平衡的关键调控区域,结缕草根系对Ca^(2+)没有区隔作用或区隔不明显。另外,耐盐结缕草Z011可能具有不同于其他3个材料的H+调控机制。Taking salt tolerant Zoysia japonica Steud.Z011 and Z015 and salt sensitive Z.japonica Z004 and Z103 as research materials,changes of Ca^(2+)and H+flow in different root zones and distribution of Ca^(2+)in roots of Z.japonica under NaCl stress(200 mmol·L-1 NaCl)were analyzed by using non-invasive micro-test technique and X-ray energy spectrometer.The results show that under the condition of the control,Ca^(2+)in meristem zone,elongation zone and maturation zone of root tips of four Z.japonica materials are mainly efflux,in which the average of Ca^(2+)flux in meristem zone is obviously higher than those in elongation zone and maturation zone,and the average of Ca^(2+)flux in meristem zone of salt tolerant Z.japonica is obviously higher than that of salt sensitive Z.japonica;after NaCl stress,in general,Ca^(2+)in three root zones of salt tolerant Z.japonica turn from efflux into influx,while only Ca^(2+)in maturation zone of root tips of salt sensitive Z.japonica turn from efflux into influx,and more Ca^(2+)influx is involved in ion regulation in salt tolerant Z.japonica than in salt sensitive Z.japonica.H+flow of salt tolerant Z.japonica Z011 is remarkably different from those of the other three materials,and under the condition of the control,H+in three root zones of Z011 are all influx,while H+in three root zones of the other three materials are weak influx or efflux,and their averages of H+flux are evidently lower than that of Z011;after NaCl stress,H+influx in three root zones of Z011 evidently decrease,while H+influx or efflux in three root zones of the other three materials also decrease,but the differences are not significant.The scan results show that in general,after NaCl stress,the relative contents of Ca^(2+)in epidermis,cortex and stele of roots of four Z.japonica materials significantly decrease,and show a tendency to first decrease and then increase with the elongation of treatment time;there are differences in relative contents of Ca^(2+)among epidermis,cortex and stele of roots of the same materia

关 键 词：结缕草 耐盐性 CA^(2+) H^(+) 离子流 离子分布 

分 类 号：Q945.78[生物学—植物学] S564.4[农业科学—作物学]