雪白睡莲花活性成分对奶牛乳腺炎主要致病菌抑菌和抗炎作用研究  

Effects of Effective Components of Nymphaea candida on Antibacterial and Anti-inflammatory Activities of Major Pathogenic Bacteria in Cow Mastitis

作　　者：张伟[1] 朱怡萱 彭菲 刘诗芃 向微微 赛福丁·阿不拉[1] 阿得力江·吾斯曼 刘丹丹 ZHANG Wei;ZHU Yi-xuan;PENG fei;LIU Shi-peng;XIANG Wei-wei;Saifuding·Abula;Adelijiang·Wusiman;LIU Dan-dan(Institute of Veterinary Medicine,Xinjiang Agricultural University,Urumqi,Xinjiang ,830052,China;Manzhouli Customs Technology Center,Manzhouli,Inner Mongolia,021400,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]满洲里海关技术中心,内蒙古满洲里021400

出　　处：《动物医学进展》2022年第6期63-68,共6页Progress In Veterinary Medicine

基　　金：2018年南农-新农联合基金项目(H)(18NN-XNLHJJ);新疆维吾尔自治区自然科学基金项目(2018D01B19);新疆农业大学校前期项目(XJAU201716);新疆维吾尔自治区研究生科研创新项目(XJ2021G180);2021年新疆农业大学校级大学生创新项目;新疆维吾尔自治区天山创新团队(2020D14005)。

摘　　要：为了研究雪白睡莲花多糖、黄酮对奶牛乳腺炎主要致病菌的抑菌和抗炎作用,采用超声提取法提取雪白睡莲花活性成分(多糖、黄酮),然后采用牛津杯法结合倍比稀释法测定其抑菌圈、最低抑菌浓度(MIC)及最低杀菌浓度(MBC),研究其对奶牛乳腺炎主要致病菌(大肠埃希氏菌、金黄色葡萄球菌、链球菌)的抑制效果;同时,通过二甲苯致小鼠耳廓肿胀试验,观察其抗炎作用。结果显示,超声波提取雪白睡莲花多糖、黄酮的提取率分别为51.78%和12.00%;体外抑菌试验可知,金黄色葡萄球菌对多糖高敏,抑菌圈直径为23.01 mm±0.43 mm;雪白睡莲花黄酮对3种致病菌均有抑制作用,且可达高敏或中敏,抑菌圈直径为(13.30±0.27)mm~(24.07±0.72)mm;抗炎试验表明,与空白对照组相比,雪白睡莲花多糖、黄酮均能极显著降低二甲苯引起的小鼠耳廓肿胀(P<0.01),肿胀抑制率分别为83.11%和65.15%。结果表明,雪白睡莲花多糖和黄酮均具有较好的抑菌抗炎作用,研究结果可为雪白睡莲花活性成分的开发利用奠定一定的理论基础。In order to study the antibacterial and anti-inflammatory effects of polysaccharides and flavonoids from Nymphaea candida on the main pathogenic bacteria of cow mastitis,the active components(polysaccharides and flavonoids)of N.candida were extracted by ultrasonic extraction in this study,and then their inhibition zones,minimum inhibitory concentrations(MICs)and minimum bactericidal concentrations(MBCs)were determined by Oxford cup method combined with doubling dilution method to study their inhibitory effects on the main pathogenic bacteria of cow mastitis(E.coli,S.aureus and Streptococci);meanwhile,their anti-inflammatory effects were observed by xylene-induced mouse auricle swelling test.The results showed that the extraction rates of polysaccharide and flavone were 51.78%and 12.00%,respectively;in vitro antibacterial test showed that S.aureus was highly sensitive to polysaccharide,with the diameter of inhibition zone of(23.01±0.43)mm;flavone of N.candida inhibited all three pathogenic bacteria,and was highly sensitive or moderately sensitive,with the diameter of inhibition zone of(13.30±0.27)mm-(24.07±0.72)mm;anti-inflammatory test showed that compared with the blank control group,polysaccharide and flavone of N.candida could significantly reduce xylene-induced auricle swelling in mice(P<0.01),with the swelling inhibition rates of 83.11%and 65.15%,respectively.The results showed that both polysaccharides and flavonoids had good antibacterial and anti-inflammatory effects,and the results could lay a theoretical foundation for the development and utilization of active components of N.candida.

关 键 词：雪白睡莲花 超声波提取 奶牛乳腺炎 体外抑菌 抗炎活性 

分 类 号：S853.74[农业科学—临床兽医学] S857.26[农业科学—兽医学]