奶山羊乳房炎二联灭活疫苗的制备及其免疫效果评估  

Preparation and Immune Effect Evaluation of Bivalent Inactivated Vaccine against Mastitis in Dairy Goats

作　　者：刘程媛 贾延德 张富强 纪甜甜 王天星 姜悦才 赵慧英[1] 陈德坤[1] 马文涛 LIU Cheng-yuan;JIA Yan-de;ZHANG Fu-qiang;JI Tian-tian;WANG Tian-xing;JIANG Yue-cai;ZHAO Hui-ying;CHEN De-kun;MA Wen-tao(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China;Animal Husbandry and Veterinary Station in Huangzhong District,Xining City,Xining,Qinghai,810099,China;Shaanxi Qianyang Saneng Dairy Goat Development Co.,Ltd.,Qianyang,Shaanxi,721100,China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]西宁市湟中区畜牧兽医站,青海西宁810099 [3]陕西千阳莎能奶山羊发展有限公司,陕西千阳721100

出　　处：《动物医学进展》2022年第7期24-29,共6页Progress In Veterinary Medicine

基　　金：陕西省重点产业创新链(群)项目(2020ZDLNY02-06);青海省重点研发与转化计划项目(2018-NK-125);畜牧专项资金项目(XN01)。

摘　　要：为评估自制乳房炎灭活疫苗的免疫效果,以乳房炎山羊乳中分离出的金黄色葡萄球菌和大肠埃希氏菌为材料,经甲醛灭活后与铝胶盐-转移因子佐剂混合,制成奶山羊乳房炎二联灭活疫苗。对泌乳期奶山羊进行2次间隔为15 d的颈部皮下注射接种,每次剂量均为2 mL。在二免后第0、15、35、60天检测乳清和血清中大肠埃希氏菌和金黄色葡萄球菌的抗体效价,并统计隐性乳房炎的检出率。结果显示,两次免疫后奶山羊乳清和血清中的抗体效价均显著升高(P<0.05),在二免后第15天达到峰值,约为对照组抗体效价的32倍;隐性乳房炎检出率显著下降(P<0.01),由免疫前的50%,在二免后第35天下降到了6.25%。结果表明,制备的奶山羊乳房炎二联灭活疫苗不仅能刺激免疫羊产生较为强烈的体液免疫应答,而且对隐性乳房炎有一定的治疗效果。To evaluate the immune effect of the inactivated mastitis vaccine,Staphylococcus aureus and Escherichia coli isolated from mastitis goat milk were inactivated by formaldehyde,and thoroughly mixed with aluminum colloidal salt-transfer factor adjuvant to make the vaccine.Lactating dairy goats were vaccinated with 2 mL of the vaccine subcutaneously via neck twice with an interval of 15 d.The antibody titers in milk and serum were detected on the 0,15,35,60 days after the second immunization,and the detection rate of recessive mastitis was counted.The results showed that the antibody titers in milk and serum were significantly increased after two immunizations(P<0.05),reached the peak on the 15th day after the second immunization,which was about 32 times of the antibody titers in the control group;and the detection rate of recessive mastitis was significantly reduced(P<0.01),decreased from 50%before immunization to 6.25%on the 35th day after the second immunization.It showed that the vaccine prepared in this study can not only stimulate a strong humoral immune response,but also have a certain therapeutic effect on recessive mastitis.

关 键 词：奶山羊 乳房炎 病原菌 灭活疫苗 抗体效价 

分 类 号：S857.26[农业科学—临床兽医学] S858.26[农业科学—兽医学]