阿魏菇粗多糖体外脾细胞调节功能及体内疫苗佐剂功能的研究  

Study on Splenocyte Regulation Function in Vitro and Vaccine Adjuvant Function in Vivo of Pleurotus ferulae Crude Polysaccharide

作　　者：史茜[1,2] 胡都斯·艾尔肯 龙志新 王科珂 蒋刚强 史向向 张富春 SHI Qian;HUDUSI-Aierken;LONG Zhi-xin;WANG Ke-ke;JIANG Gang-qiang;SHI Xiang-xiang;ZHANG Fu-chun(College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang,830046,China;Urumqi Customs Technology Center,Urumqi,Xinjiang,830063,China;Tuergate Customs,Tuergate,Xinjiang,845452,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]乌鲁木齐海关技术中心,新疆乌鲁木齐830063 [3]吐尔尕特海关,新疆吐尔尕特845452

出　　处：《动物医学进展》2022年第7期57-63,共7页Progress In Veterinary Medicine

基　　金：新疆维吾尔自治区科技支疆项目(201591134)。

摘　　要：为研究阿魏菇粗多糖(PFCP)体外对小鼠脾细胞增殖的作用和体内对口蹄疫灭活疫苗的佐剂功能,MTT法检测PFCP最大无毒浓度,PFCP与Con A、LPS联用刺激小鼠脾细胞增殖的能力,ELISA检测细胞培养上清中IL-4与IFN-γ含量。PFCP联合口蹄疫病毒灭活抗原皮下注射免疫接种小鼠,ELISA检测IgG生成情况,流式细胞术分析免疫后小鼠脾脏T淋巴细胞亚群比例。结果显示,PFCP安全性好,体外可协同ConA与LPS促进小鼠脾细胞的增殖,增加IL-4与IFN-γ的分泌量。体内可增强口蹄疫病毒特异性IgG的水平,增加CD3+CD4+T、CD3+CD8+T淋巴细胞的数量。表明PFCP对小鼠脾细胞增殖具有较好的促进作用,且具有作为口蹄疫灭活疫苗佐剂的潜力。In order to study the effects of Pleurotus ferulae crude polysaccharide(PFCP)on the proliferation of mouse splenocytes in vitro and the adjuvant function of inactivated foot-and-mouth disease virus inactivated vaccine in vivo,MTT assay was used to detect the maximum non-toxic concentration of PFCP and the ability of PFCP combined with Con A and LPS to stimulate the proliferation of mouse splenocytes.IL-4 and IFN-γcontents in cell culture supernatant were detected by ELISA.Mice were immunized subcutaneously with PFCP combined with foot-and-mouth disease virus inactivated antigen.The production of specific antibody was detected by ELISA,and the proportion of spleen T lymphocyte subsets was analyzed by flow cytometry.The results showed that PFCP was safe and could cooperate with ConA and LPS to promote the proliferation of mouse splenocytes and increase IL-4 and IFN-γsecretions in vitro.It could enhance the expression of foot-and-mouth disease virus inactivated antigen-specific IgG and increase the number of CD3+CD4+T and CD3+CD8+T lymphocytes.The results showed that PFCP can promote the proliferation of splenocytes in mice,and has the potential to be used as adjuvant of inactivated foot-and-mouth disease vaccine.

关 键 词：阿魏菇 多糖 脾细胞 口蹄疫 佐剂 

分 类 号：S852.42[农业科学—基础兽医学] S852.659.1[农业科学—兽医学]